Literature DB >> 2945215

Cold labelled substrate and estimation of cholesterol esterification rate in lecithin cholesterol acyltransferase radioassay.

M Dobiásová, M Schützová.   

Abstract

A simple and reproducible radioassay of lecithin cholesterol acyltransferase (LCAT E.C. 2.3.1.43) activity is described. The method is based on cold labelling of the serum, plasma or other LCAT and lipoproteins containing fluids with a trace of 14C-cholesterol spread in ready made sorbent discs. Since the procedure minimizes the chemical, heating and time consuming steps, it may be particularly suitable for routine investigation of the defects of cholesterol kinetics and also for experimental studies.

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Year:  1986        PMID： 2945215

Source DB:  PubMed          Journal:  Physiol Bohemoslov        ISSN： 0369-9463


  7 in total

1.  Uptake and metabolism of lipoprotein-X in mesangial cells.

Authors:  E G Lynn; P C Choy; A Magil; K O
Journal:  Mol Cell Biochem       Date:  1997-10       Impact factor: 3.396

2.  Effect of methylprednisolone therapy on lipoprotein metabolism in human nephrotic syndrome.

Authors:  L Kuzemková; J Stríbrná; J Kovár; R Poledne; K Matousovic
Journal:  Int Urol Nephrol       Date:  1989       Impact factor: 2.370

3.  A unique genetic and biochemical presentation of fish-eye disease.

Authors:  J A Kuivenhoven; E J van Voorst tot Voorst; H Wiebusch; S M Marcovina; H Funke; G Assmann; P H Pritchard; J J Kastelein
Journal:  J Clin Invest       Date:  1995-12       Impact factor: 14.808

4.  An intronic mutation in a lariat branchpoint sequence is a direct cause of an inherited human disorder (fish-eye disease).

Authors:  J A Kuivenhoven; H Weibusch; P H Pritchard; H Funke; R Benne; G Assmann; J J Kastelein
Journal:  J Clin Invest       Date:  1996-07-15       Impact factor: 14.808

5.  Fish eye syndrome: a molecular defect in the lecithin-cholesterol acyltransferase (LCAT) gene associated with normal alpha-LCAT-specific activity. Implications for classification and prognosis.

Authors:  H G Klein; S Santamarina-Fojo; N Duverger; M Clerc; M F Dumon; J J Albers; S Marcovina; H B Brewer
Journal:  J Clin Invest       Date:  1993-07       Impact factor: 14.808

6.  Lecithin:cholesterol acyltransferase: role of N-linked glycosylation in enzyme function.

Authors:  K O; J S Hill; X Wang; R McLeod; P H Pritchard
Journal:  Biochem J       Date:  1993-09-15       Impact factor: 3.857

7.  Lecithin:Cholesterol Acyltransferase Activation by Sulfhydryl-Reactive Small Molecules: Role of Cysteine-31.

Authors:  Lita A Freeman; Stephen J Demosky; Monika Konaklieva; Rostislav Kuskovsky; Angel Aponte; Alice F Ossoli; Scott M Gordon; Ross F Koby; Kelly A Manthei; Min Shen; Boris L Vaisman; Robert D Shamburek; Ajit Jadhav; Laura Calabresi; Marjan Gucek; John J G Tesmer; Rodney L Levine; Alan T Remaley
Journal:  J Pharmacol Exp Ther       Date:  2017-06-02       Impact factor: 4.030
  7 in total

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