Magnetic combined cross-linked enzyme aggregates (Combi-CLEAs) for cofactor regeneration in the synthesis of chiral alcohol.

Magnetic Fe3O4 nanoparticles were prepared and embedded into the Combi-CLEAs to produce the magnetic Combi-CLEAs in this work. The process for magnetic Combi-CLEAs preparation was optimized, and its properties were investigated. The optimum temperature, thermal stability and optimum pH of magnetic Combi-CLEAs were similar to those of Combi-CLEAs. The catalytic performance of magnetic Combi-CLEAs was tested with the biosynthesis of (S)-ethyl 4-chloro-3-hydroxybutyrate ((S)-CHBE). Magnetic Combi-CLEAs could tolerate higher substrate concentration in the biphasic system. The catalytic efficiency and long-term operational stability of magnetic Combi-CLEAs were obviously superior to those of Combi-CLEAs in both aqueous and biphasic systems. Embedding of magnetic Fe3O4 nanoparticles endowing rigidity contributed to these improvements. Furthermore, the preparation of magnetic Combi-CLEAs was easy, and its recovery during multiple batches of reactions could be fulfilled by magnetic field. Aforementioned advantages make the magnetic Combi-CLEAs hold obvious potential for industrial application.