Ping Gong1,2, Bingwei Shi3, Juan Wang3, Peixia Cao1, Zhenyu Diao2, Yuji Wang1, Yali Hu2, Shuping Li1. 1. a Department of Obstetrics and Gynecology , Changzhou Hospital of Traditional Chinese Medicine, Nanjing University of Chinese Medicine , Changzhou , China. 2. b Department of Obstetrics and Gynecology , Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine , Nanjing , China. 3. c Department of Laboratory Medicine , Changzhou Hospital of Traditional Chinese Medicine , Changzhou , China.
Abstract
OBJECTIVES: This study aimed to investigate the Th1/Th2 cells in peripheral blood of PCOS patients, and assess the potential correlation between Th1/Th2 imbalance and obesity. METHODS: Thirty-nine PCOS patients and 23 age-matched controls were enrolled. The PBMCs were obtained before pharmacological intervention in women with or without PCOS. The profiles of Th1 (IFN-γ) and Th2 (IL-4) cytokines of CD3+CD- T lymphocyte subsets were analyzed by flow cytometry. Plasma sex hormones including E2, T, FSH, LH, and FINS, FPG were measured, together with BMI, WC, LH/FSH, E2/T and HOMA-IR index being calculated. Association between Th1/Th2 imbalance and BMI, WC were evaluated. RESULTS: The proportion of Th1 cells and Th1/Th2 ratio were significantly higher in PCOS patients than those in controls, accompanied by elevated T, LH, LH/FSH, FINS, HOMA-IR index and reduced E2/T. The Th1/Th2 ratio was increased when BMI and WC were enhanced in PCOS. Moreover, the significant difference of Th1/Th2 ratio was observed between WC subgroups of PCOS. CONCLUSIONS: It is concluded that Th1 type immunity is predominant in systemic immunization of PCOS patients. Th1/Th2 immune imbalance is connected with obesity, especially abdominal obesity, and may be one of the underlying mechanism for the pathogenesis of PCOS.
OBJECTIVES: This study aimed to investigate the Th1/Th2 cells in peripheral blood of PCOSpatients, and assess the potential correlation between Th1/Th2 imbalance and obesity. METHODS: Thirty-nine PCOSpatients and 23 age-matched controls were enrolled. The PBMCs were obtained before pharmacological intervention in women with or without PCOS. The profiles of Th1 (IFN-γ) and Th2 (IL-4) cytokines of CD3+CD- T lymphocyte subsets were analyzed by flow cytometry. Plasma sex hormones including E2, T, FSH, LH, and FINS, FPG were measured, together with BMI, WC, LH/FSH, E2/T and HOMA-IR index being calculated. Association between Th1/Th2 imbalance and BMI, WC were evaluated. RESULTS: The proportion of Th1 cells and Th1/Th2 ratio were significantly higher in PCOSpatients than those in controls, accompanied by elevated T, LH, LH/FSH, FINS, HOMA-IR index and reduced E2/T. The Th1/Th2 ratio was increased when BMI and WC were enhanced in PCOS. Moreover, the significant difference of Th1/Th2 ratio was observed between WC subgroups of PCOS. CONCLUSIONS: It is concluded that Th1 type immunity is predominant in systemic immunization of PCOSpatients. Th1/Th2 immune imbalance is connected with obesity, especially abdominal obesity, and may be one of the underlying mechanism for the pathogenesis of PCOS.
Entities:
Keywords:
Body Mass Index; Polycystic ovary syndrome; Th1/Th2 immune imbalance; obesity; waist circumference