Ozan Luay Abbas1, Orhan Özatik2, Zeynep Burçin Gönen3, Serdal Öğüt4, Fikriye Yasemin Özatik5, Hasan Salkın6, Ahmet Musmul7. 1. Ahi Evran University, Faculty of Medicine, Department of Plastic, Reconstructive and Aesthetic Surgery, Kırşehir, Turkey. 2. Dumlupınar University, Faculty of Medicine, Department of Histology and Embryology, Kütahya, Turkey. 3. Erciyes University, Gen Kök Genome and Stem Cell Center, Kayseri, Turkey. 4. Adnan Menderes University, Faculty of Health Science, Department of Nutrition and Dietetics, Aydın, Turkey. 5. Dumlupınar University, Faculty of Medicine, Department of Pharmacology, Kütahya, Turkey. 6. Beykent University, Vocational School, Department of Medical Services and techniques, Istanbul, Turkey. 7. Osmangazi University, Faculty of Medicine, Department of Biostatistics, Eskişehir, Turkey.
Abstract
Introduction: The implantation of mesenchymal stem cells (MSCs) has been shown to exert benefits for the survival of the zone-of-stasis. However, the clinical experience indicates the importance of selecting the right source and type of stem cells. Therefore, we planned the current study to perform a quantitative comparison of MSCs isolated from three different sources to provide information useful in selection of the optimal source and to see whether critical mechanisms are conserved between different populations. Methods: The protective effects of MSCs derived from bone marrow, adipose tissue and dental pulp were compared in a rat model of thermal trauma. The stasis zones were evaluated 72 h after the burn using histochemistry, immunohistochemistry and biochemistry. Results: Gross evaluation of burn wounds revealed that the differences between the mean percentages of the calculated necrotic areas weren't statistically significant. Semi-quantitative grading of the histopathological findings revealed that there were no significant differences between damage scores. Immunohistochemical assessment of apoptotic and necrotic cell deaths revealed that the differences between the mean numbers of apoptotic and necrotic cells weren't statistically significant. Myeloperoxidase activity was found to be significantly lower in the adipose tissue group. Biochemical and immunohistochemical assessment of tissue malondialdehyde revealed that the differences between the groups weren't statistically significant. Finally, the number of neo-vessels in the dental pulp group was found to be significantly higher. Conclusion: Our findings suggest that bone marrow, adipose tissue and dental pulp may serve as a universal donor MSC source for the prevention of burn wound progression.
Introduction: The implantation of mesenchymal stem cells (MSCs) has been shown to exert benefits for the survival of the zone-of-stasis. However, the clinical experience indicates the importance of selecting the right source and type of stem cells. Therefore, we planned the current study to perform a quantitative comparison of MSCs isolated from three different sources to provide information useful in selection of the optimal source and to see whether critical mechanisms are conserved between different populations. Methods: The protective effects of MSCs derived from bone marrow, adipose tissue and dental pulp were compared in a rat model of thermal trauma. The stasis zones were evaluated 72 h after the burn using histochemistry, immunohistochemistry and biochemistry. Results: Gross evaluation of burn wounds revealed that the differences between the mean percentages of the calculated necrotic areas weren't statistically significant. Semi-quantitative grading of the histopathological findings revealed that there were no significant differences between damage scores. Immunohistochemical assessment of apoptotic and necrotic cell deaths revealed that the differences between the mean numbers of apoptotic and necrotic cells weren't statistically significant. Myeloperoxidase activity was found to be significantly lower in the adipose tissue group. Biochemical and immunohistochemical assessment of tissue malondialdehyde revealed that the differences between the groups weren't statistically significant. Finally, the number of neo-vessels in the dental pulp group was found to be significantly higher. Conclusion: Our findings suggest that bone marrow, adipose tissue and dental pulp may serve as a universal donor MSC source for the prevention of burn wound progression.
Entities:
Keywords:
Burn; adipose tissue; bone marrow; dental pulp; mesenchymal stem cell; stasis zone
Authors: Dina Rady; Marwa M S Abbass; Aiah A El-Rashidy; Sara El Moshy; Israa Ahmed Radwan; Christof E Dörfer; Karim M Fawzy El-Sayed Journal: Stem Cells Int Date: 2020-08-11 Impact factor: 5.443
Authors: Anastasia Shpichka; Denis Butnaru; Evgeny A Bezrukov; Roman B Sukhanov; Anthony Atala; Vitaliy Burdukovskii; Yuanyuan Zhang; Peter Timashev Journal: Stem Cell Res Ther Date: 2019-03-15 Impact factor: 6.832
Authors: Alen Palackic; Jayson W Jay; Robert P Duggan; Ludwik K Branski; Steven E Wolf; Naseem Ansari; Amina El Ayadi Journal: Medicina (Kaunas) Date: 2022-07-11 Impact factor: 2.948