Mahmoud A Ali1, Marwa Matboli2, Nashwa El-Khazragy3, Osama Saber4, Sarah El-Nakeep5, Hana M Abdelzaher6, Ayman El-Sayed Shafei1, Randa Mostafa1. 1. Department of Biomedical Research, Armed Forces College of Medicine (AFCM), Cairo, 11774, Egypt. 2. Department of Medical Biochemistry & Molecular Biology, Ain Shams Faculty of Medicine Research Center (Masri), 11778, Eygpt. 3. Department of Clinical Pathology, Oncology Diagnostic Unit, Faculty of Medicine, Ain Shams University, Cairo, 11778, Egypt. 4. Armed Forces College of Medicine (AFCM), Cairo, 11774, Egypt. 5. Hepatology & Gastroenterology Unit, Internal Medicine Department, Faculty of Medicine, Ain Shams University, Cairo, 11778, Egypt. 6. Faculty of Biotechnology, October University for Modern Sciences & Arts, Cairo, 12585, Egypt.
Abstract
AIM: We aimed to examine the statistical association between serum expression of miRNA 661 (miR-661) and ATG-4B mRNA and hepatocellular carcinoma (HCC) based on in silico data analysis followed by clinical validation. PATIENTS & METHODS: Quantitative reverse-transcriptase real-time PCR was used to examine the expression of miR-661 and ATG-4B mRNA in the sera of HCC patients versus control. RESULTS: The expression of miR-661 and ATG-4B mRNA was positive in 97.14 and 77.14%, respectively, in HCC patients. The survival analysis showed that ATG-4B mRNA was an independent prognostic factor. CONCLUSION: Our data are the first report of its kind regarding the considerable clinical significance of miR-661 and ATG-4B mRNA in HCC patients.
AIM: We aimed to examine the statistical association between serum expression of miRNA 661 (miR-661) and ATG-4B mRNA and hepatocellular carcinoma (HCC) based on in silico data analysis followed by clinical validation. PATIENTS & METHODS: Quantitative reverse-transcriptase real-time PCR was used to examine the expression of miR-661 and ATG-4B mRNA in the sera of HCC patients versus control. RESULTS: The expression of miR-661 and ATG-4B mRNA was positive in 97.14 and 77.14%, respectively, in HCC patients. The survival analysis showed that ATG-4B mRNA was an independent prognostic factor. CONCLUSION: Our data are the first report of its kind regarding the considerable clinical significance of miR-661 and ATG-4B mRNA in HCC patients.