Josep Marí-Alexandre1, Moises Barceló-Molina1, Jorge Sanz-Sánchez2, Pilar Molina3, Jennifer Sancho3, Yolanda Abellán3, María Luisa Santaolaria-Ayora4, Juan Giner3, Luis Martínez-Dolz2, Amparo Estelles1, Aitana Braza-Boïls5, Esther Zorio6. 1. Unidad de Cardiopatías Familiares, Muerte Súbita y Mecanismos de Enfermedad (CaFaMuSMe), Instituto de Investigación Sanitaria La Fe, Valencia, Spain. 2. Servicio de Cardiología, Hospital Universitario y Politécnico La Fe, Valencia, Spain. 3. Unidad de Cardiopatías Familiares, Muerte Súbita y Mecanismos de Enfermedad (CaFaMuSMe), Instituto de Investigación Sanitaria La Fe, Valencia, Spain; Servicio de Patología, Instituto de Medicina Legal y Ciencias Forenses, Valencia, Spain. 4. Servicio de Análisis Clínicos, Hospital Universitario Dr. Peset, Valencia, Spain. 5. Unidad de Cardiopatías Familiares, Muerte Súbita y Mecanismos de Enfermedad (CaFaMuSMe), Instituto de Investigación Sanitaria La Fe, Valencia, Spain. Electronic address: aitana_braza@iislafe.es. 6. Unidad de Cardiopatías Familiares, Muerte Súbita y Mecanismos de Enfermedad (CaFaMuSMe), Instituto de Investigación Sanitaria La Fe, Valencia, Spain; Servicio de Cardiología, Hospital Universitario y Politécnico La Fe, Valencia, Spain. Electronic address: zorio_est@gva.es.
Abstract
INTRODUCTION AND OBJECTIVES: An increased epicardial adipose tissue (EAT) thickness has become a new risk factor for coronary heart disease (CHD). We aimed to study the role of EAT dysfunction as a CHD marker by focusing on its thickness and microRNA (miRNA) expression profile, and the potential factors possibly influencing them. METHODS: One hundred and fifty-five CHD sudden cardiac death victims and 84 non-CHD-sudden death controls were prospectively enrolled at autopsy. A representative subset underwent EAT thickness measurements and EAT miRNA expression profiling. RESULTS: Epicardial adipose tissue thickness was increased and allowed an accurate diagnosis of patient status (among other measurements, EAT score area under the curve 0.718, P < .001). Epicardial adipose tissue from patients showed 14 up- and 14 down-regulated miRNAs and miR-34a-3p, -34a-5p, -124-3p, -125a-5p, 628-5p, -1303 and -4286 were validated by quantitative real-time polymerase chain reaction. Patients exhibited higher EAT levels of miR-34a-3p and -34a-5p than controls (with a positive trend considering EAT from coronaries without stenosis, with stable stenosis and complicated plaques) and correlated with age only in controls. The mild positive correlation between liver and EAT miR-34a-5p levels in patients (r = 0.295, P = .020) dramatically increased in EAT from complicated plaques (r = 0.799, P = .017). Similar correlations were observed for high-sensitivity-C-reactive protein levels and miR-34a-5p levels both in EAT and liver extracts. CONCLUSIONS: Increased age-independent levels of miR-34a-3p and -34a-5p characterize the EAT miRNA expression profile of CHD regardless of EAT thickness, anthropometric parameters, and the presence of underlying atherosclerotic plaques.
INTRODUCTION AND OBJECTIVES: An increased epicardial adipose tissue (EAT) thickness has become a new risk factor for coronary heart disease (CHD). We aimed to study the role of EAT dysfunction as a CHD marker by focusing on its thickness and microRNA (miRNA) expression profile, and the potential factors possibly influencing them. METHODS: One hundred and fifty-five CHD sudden cardiac death victims and 84 non-CHD-sudden death controls were prospectively enrolled at autopsy. A representative subset underwent EAT thickness measurements and EAT miRNA expression profiling. RESULTS: Epicardial adipose tissue thickness was increased and allowed an accurate diagnosis of patient status (among other measurements, EAT score area under the curve 0.718, P < .001). Epicardial adipose tissue from patients showed 14 up- and 14 down-regulated miRNAs and miR-34a-3p, -34a-5p, -124-3p, -125a-5p, 628-5p, -1303 and -4286 were validated by quantitative real-time polymerase chain reaction. Patients exhibited higher EAT levels of miR-34a-3p and -34a-5p than controls (with a positive trend considering EAT from coronaries without stenosis, with stable stenosis and complicated plaques) and correlated with age only in controls. The mild positive correlation between liver and EAT miR-34a-5p levels in patients (r = 0.295, P = .020) dramatically increased in EAT from complicated plaques (r = 0.799, P = .017). Similar correlations were observed for high-sensitivity-C-reactive protein levels and miR-34a-5p levels both in EAT and liver extracts. CONCLUSIONS: Increased age-independent levels of miR-34a-3p and -34a-5p characterize the EAT miRNA expression profile of CHD regardless of EAT thickness, anthropometric parameters, and the presence of underlying atherosclerotic plaques.
Authors: Ursula Paula Reno Soci; Bruno Raphael Ribeiro Cavalcante; Alex Cleber Improta-Caria; Leonardo Roever Journal: Front Cell Dev Biol Date: 2022-07-04
Authors: Yuri Levin-Schwartz; Paul Curtin; Daniel Flores; Vasily N Aushev; Marcela Tamayo-Ortiz; Katherine Svensson; Ivan Pantic; Guadalupe Estrada-Gutierrez; María L Pizano-Zárate; Chris Gennings; Lisa M Satlin; Andrea A Baccarelli; Martha M Tellez-Rojo; Robert O Wright; Alison P Sanders Journal: Epigenomics Date: 2021-02-26 Impact factor: 4.778
Authors: Valmore Bermúdez; Pablo Durán; Edward Rojas; María P Díaz; José Rivas; Manuel Nava; Maricarmen Chacín; Mayela Cabrera de Bravo; Rubén Carrasquero; Clímaco Cano Ponce; José Luis Górriz; Luis D Marco Journal: Front Endocrinol (Lausanne) Date: 2021-09-15 Impact factor: 5.555