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Literature DB >> 29437997

Fluorescence In Situ Hybridization of Cryosectioned Xenopus Oocytes.

Abstract

Xenopus laevis oocytes are widely used to study mechanisms of RNA function and biogenesis. While the large size of Xenopus oocytes is amenable to both biochemical and imaging approaches, the relative opacity of the yolk-rich cytoplasm has limited high-resolution imaging of endogenous RNAs. Here, we present a protocol that combines multi-probe fluorescence in situ hybridization with cryosectioning to provide a highly sensitive means of imaging endogenous oocyte RNAs.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
RNA

Year: 2018 PMID： 29437997 PMCID： PMC5932210 DOI： 10.1101/pdb.prot097030

Source DB: PubMed Journal: Cold Spring Harb Protoc ISSN： 1559-6095

17 in total

Review 1. Electron microscopy, immunostaining, cytoskeleton visualization, in situ hybridization, and three-dimensional reconstruction of Xenopus oocytes.

Authors: Szczepan M Bilinski; Mariusz K Jaglarz; Matthew T Dougherty; Malgorzata Kloc
Journal: Methods Date: 2009-12-16 Impact factor: 3.608

2. Potential structural role of non-coding and coding RNAs in the organization of the cytoskeleton at the vegetal cortex of Xenopus oocytes.

Authors: Malgorzata Kloc; Katarzyna Wilk; Diana Vargas; Yuri Shirato; Szczepan Bilinski; Laurence D Etkin
Journal: Development Date: 2005-07-06 Impact factor: 6.868

3. Isolation of Xenopus Oocytes.

Authors: Karen Newman; Tristan Aguero; Mary Lou King
Journal: Cold Spring Harb Protoc Date: 2018-02-01

4. The Xenopus ELAV protein ElrB represses Vg1 mRNA translation during oogenesis.

Authors: Lucy J Colegrove-Otero; Agathe Devaux; Nancy Standart
Journal: Mol Cell Biol Date: 2005-10 Impact factor: 4.272

5. Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.

Authors: L L McGrew; E Dworkin-Rastl; M B Dworkin; J D Richter
Journal: Genes Dev Date: 1989-06 Impact factor: 11.361

Fluorescence In Situ Hybridization of Cryosectioned Xenopus Oocytes.

Review 1. Electron microscopy, immunostaining, cytoskeleton visualization, in situ hybridization, and three-dimensional reconstruction of Xenopus oocytes.

2. Potential structural role of non-coding and coding RNAs in the organization of the cytoskeleton at the vegetal cortex of Xenopus oocytes.

3. Isolation of Xenopus Oocytes.

4. The Xenopus ELAV protein ElrB represses Vg1 mRNA translation during oogenesis.

5. Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.

6. Translocation of a localized maternal mRNA to the vegetal pole of Xenopus oocytes.

7. Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes.

8. CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.

9. Xenopus Staufen is a component of a ribonucleoprotein complex containing Vg1 RNA and kinesin.

10. Imaging individual mRNA molecules using multiple singly labeled probes.

1. Using the Xenopus Oocyte Toolbox.

2. Cryosectioning and Immunostaining of Xenopus Embryonic Tissues.

3. L-bodies are RNA-protein condensates driving RNA localization in Xenopus oocytes.