Draft Genome Sequence of Monaibacterium marinum C7T, Isolated from Seawater from the Menai Straits, Wales, United Kingdom.

Here, we report the draft genome sequence of Monaibacterium marinum C7T, a strain that represents a new member of the Roseobacter clade of the family Rhodobacteraceae (Alphaproteobacteria). The genome size of Monaibacterium marinum C7T is 3.7 Mb (3,734,267 bp), with a G+C content of 58.86%.

GENOME ANNOUNCEMENT

The Roseobacter clade within the Rhodobacteraceae (Alphaproteobacteria) represents a distinct and diverse group of microorganisms that constitute up to 25% of the coastal marine bacteria (1, 2). The study of the genomic features of members of this clade provides an insight into their ecology, physiology, niche specificity, and adaptation to the environmental stimuli.

The new strain Monaibacterium marinum C7T was isolated from a seawater sample taken from Menai Straits (Wales, UK) and represents a distinct lineage within the Roseobacter clade of the family Rhodobacteraceae within the Alphaproteobacteria (3). Strain C7T is an aerobic Gram-negative mesophilic nonmotile short-rod-shaped bacterium that can grow under microaerophilic conditions. It grows on ONR7a medium (4) in the range of NaCl concentrations of 0 to 9% (wt/vol), at temperatures between 4 and 31°C, and in pH range 5.5 to 9.0 with the optimum pH of 7.5.

The DNA was isolated using Meta-G-Nome DNA isolation kit (Epicentre Biotechnologies), according to the manufacturer’s instructions.

The genome of Monaibacterium marinum C7T was sequenced in the framework of the project Genomic Encyclopedia of Bacteria and Archaea, phase III, at the Department of Energy (DOE) Joint Genome Institute (JGI) using Illumina technology (5). Sequencing of a standard shotgun library with 300-bp inserts using the Illumina 9 HiSeq 2000 1-Tb platform generated 6,934,098 reads totaling 1,040.1 Mbp of sequence. Known Illumina artifacts were removed, and PhiX sequencing was done by filtering all raw Illumina sequence data using BBDuk (6). Reads with fewer than 51 bp after trimming were discarded from the analysis. The remaining reads were subjected to sequence mapping and masking using BBMAP (6) and BBMask (6), respectively. Assembly was performed using SPAdes version 3.6.2 (7). Annotation was done using the JGI-IMG Annotation Pipeline version 4.14.1.

The final draft assembly contained 27 contigs in 27 scaffolds, totaling 3.735 Mbp in size, and was based on 1,040.1 Mbp of Illumina data with a mapped coverage of 279.2-fold.

The largest contig was 1,171.7 kb. The total number of genes was 3,615, including 57 RNA genes (rRNA, 6; 5S RNA, 4; 16S RNA, 1; 23S RNA, 1; tRNA, 42; other RNA genes 3). Moreover, annotation revealed 3,558 protein-coding genes, with 2,915 having functional predictions. Analysis of the draft genome sequence of Monaibacterium marinum C7T using AromaDeg (8) and BLASTP against an in-house database (9), which contains experimentally characterized enzymes involved in the catabolism of aliphatic and aromatic compounds, predicted genes encoding two alkane hydroxylases and two P450 cytochromes (albeit, this strain was not able to grow on aliphatic hydrocarbons of carbon chain length 10 to 20 [3]), one catechol 1,2 dioxygenase for the ortho-pathway, and three carboxymuconate cycloisomerases. In addition, the gene inspection using antiSMASH (10) revealed the presence of gene clusters for the biosynthesis of terpenes, homoserine lactones, aryl propylene, and ladderanes.

Accession number(s).

This draft genome sequence of Monaibacterium marinum C7T has been deposited in DDBJ/ENA/GenBank under the accession no. OCTN01000001 to OCTN01000027. The versions described in this paper are the first versions, OCTN01000001.1 to OCTN01000027.1.