Literature DB >> 29436709

Fast and efficient genetic engineering of hematopoietic precursor cells for the study of dendritic cell migration.

Alexander Leithner1, Joerg Renkawitz1, Ingrid De Vries1, Robert Hauschild1, Hans Häcker2, Michael Sixt1.   

Abstract

Estrogen inducible Hoxb8 leads to conditional immortalization of hematopoietic precursors. These cells can be cultured and infected with the CRISPR/Cas9 system for genome editing, circumventing resource consuming generation of mouse models. The resultant cells retain their ability to differentiate into migratory dendritic cells.
© 2018 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  CCL19; CCR7; CRISPR/Cas9; Cell migration; Genome editing

Mesh：

Substances：

Year:  2018        PMID： 29436709     DOI： 10.1002/eji.201747358

Source DB:  PubMed          Journal:  Eur J Immunol        ISSN： 0014-2980            Impact factor:   5.532


  8 in total

1.  Nuclear positioning facilitates amoeboid migration along the path of least resistance.

Authors:  Jörg Renkawitz; Aglaja Kopf; Julian Stopp; Ingrid de Vries; Meghan K Driscoll; Jack Merrin; Robert Hauschild; Erik S Welf; Gaudenz Danuser; Reto Fiolka; Michael Sixt
Journal:  Nature       Date:  2019-04-03       Impact factor: 49.962

2.  Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14.

Authors:  Kathrin Tomasek; Alexander Leithner; Ivana Glatzova; Michael S Lukesch; Calin C Guet; Michael Sixt
Journal:  Elife       Date:  2022-07-26       Impact factor: 8.713

3.  Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells.

Authors:  Ann-Kathrin Weier; Mirka Homrich; Stephanie Ebbinghaus; Pavel Juda; Eliška Miková; Robert Hauschild; Lili Zhang; Thomas Quast; Elvira Mass; Andreas Schlitzer; Waldemar Kolanus; Sven Burgdorf; Oliver J Gruß; Miroslav Hons; Stefan Wieser; Eva Kiermaier
Journal:  J Cell Biol       Date:  2022-10-10       Impact factor: 8.077

4.  CRISPR/Cas9 Immunoengineering of Hoxb8-Immortalized Progenitor Cells for Revealing CCR7-Mediated Dendritic Cell Signaling and Migration Mechanisms in vivo.

Authors:  Swantje I Hammerschmidt; Kathrin Werth; Michael Rothe; Melanie Galla; Marc Permanyer; Gwendolyn E Patzer; Anja Bubke; David N Frenk; Anton Selich; Lucas Lange; Axel Schambach; Berislav Bošnjak; Reinhold Förster
Journal:  Front Immunol       Date:  2018-08-28       Impact factor: 7.561

5.  Establishment of A Reversibly Inducible Porcine Granulosa Cell Line.

Authors:  Yinshan Bai; Cui Zhu; Meiying Feng; Bo Pan; Shouquan Zhang; Xiaoshu Zhan; Huifang Chen; Bingyun Wang; Julang Li
Journal:  Cells       Date:  2020-01-08       Impact factor: 6.600

6.  WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues.

Authors:  Florian Gaertner; Patricia Reis-Rodrigues; Ingrid de Vries; Miroslav Hons; Juan Aguilera; Michael Riedl; Alexander Leithner; Saren Tasciyan; Aglaja Kopf; Jack Merrin; Vanessa Zheden; Walter Anton Kaufmann; Robert Hauschild; Michael Sixt
Journal:  Dev Cell       Date:  2021-12-16       Impact factor: 12.270

7.  Microtubules control cellular shape and coherence in amoeboid migrating cells.

Authors:  Aglaja Kopf; Jörg Renkawitz; Robert Hauschild; Irute Girkontaite; Kerry Tedford; Jack Merrin; Oliver Thorn-Seshold; Dirk Trauner; Hans Häcker; Klaus-Dieter Fischer; Eva Kiermaier; Michael Sixt
Journal:  J Cell Biol       Date:  2020-06-01       Impact factor: 10.539

8.  CAL-1 as Cellular Model System to Study CCR7-Guided Human Dendritic Cell Migration.

Authors:  Edith Uetz-von Allmen; Guerric P B Samson; Vladimir Purvanov; Takahiro Maeda; Daniel F Legler
Journal:  Front Immunol       Date:  2021-09-16       Impact factor: 7.561
  8 in total

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