Simona Slezakova1,2, Petra Borilova Linhartova1,2, Lucie Masopustova3, Jirina Bartova4, Jitka Petanova5, Pavel Kuklinek6, Antonin Fassmann1, Ladislav Dusek7, Lydie Izakovicova Holla1,2. 1. Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. 2. Clinic of Stomatology, Institution Shared with St. Anne's Faculty Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic. 3. Faculty of Science, Masaryk University, Brno, Czech Republic. 4. Institute of Clinical and Experimental Dental Medicine, General University Hospital and First Faculty of Medicine, Charles University, Prague, Czech Republic. 5. Department of Immunology and Microbiology, General University Hospital and First Faculty of Medicine, Charles University, Prague, Czech Republic. 6. Department of Clinical Immunology and Allergology, Institution Shared with St. Anne's Faculty Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic. 7. Institute of Biostatistics and Analyses, Masaryk University, Brno, Czech Republic.
Abstract
BACKGROUND: Recurrent aphthous stomatitis (RAS) is a multifactorial disease with unclear etiopathogenesis in which disturbance of immunological processes may be involved. The aim of our study was to investigate three single nucleotide polymorphisms (SNPs) rs3806265, rs4612666, rs10754558 in NOD-like receptor 3 (NLRP3), the gene encoding the component of inflammasome, in patients with RAS and healthy controls in the Czech population. METHODS: A total of 207 subjects were included in this case-control study. Sixty-four patients with RAS and 143 healthy controls were genotyped by a method based on polymerase chain reaction using 5' nuclease TaqMan® assays. Detailed anamnestic, clinical, and laboratory data were obtained from all subjects. RESULTS: The allele and genotype frequencies of NLRP3 polymorphisms (rs10754558 and rs3806265) between both groups were similar. However, statistically significant differences in NLRP3 rs4612666 genotypes between the patients with RAS and controls were found; carriers of the TT genotype had a higher risk of developing RAS than subjects with the CT+CC genotypes (OR = 14.69, 95%CI = 1.73-124.72, P = .004, Pcorr < .05). No associations between NLRP3 haplotypes and RAS were observed. CONCLUSIONS: Our study indicates that the NLRP3 rs4612666 polymorphism may be involved in the development of RAS in the Czech population.
BACKGROUND: Recurrent aphthous stomatitis (RAS) is a multifactorial disease with unclear etiopathogenesis in which disturbance of immunological processes may be involved. The aim of our study was to investigate three single nucleotide polymorphisms (SNPs) rs3806265, rs4612666, rs10754558 in NOD-like receptor 3 (NLRP3), the gene encoding the component of inflammasome, in patients with RAS and healthy controls in the Czech population. METHODS: A total of 207 subjects were included in this case-control study. Sixty-four patients with RAS and 143 healthy controls were genotyped by a method based on polymerase chain reaction using 5' nuclease TaqMan® assays. Detailed anamnestic, clinical, and laboratory data were obtained from all subjects. RESULTS: The allele and genotype frequencies of NLRP3 polymorphisms (rs10754558 and rs3806265) between both groups were similar. However, statistically significant differences in NLRP3rs4612666 genotypes between the patients with RAS and controls were found; carriers of the TT genotype had a higher risk of developing RAS than subjects with the CT+CC genotypes (OR = 14.69, 95%CI = 1.73-124.72, P = .004, Pcorr < .05). No associations between NLRP3 haplotypes and RAS were observed. CONCLUSIONS: Our study indicates that the NLRP3rs4612666 polymorphism may be involved in the development of RAS in the Czech population.
Authors: Sadeq Ali Al-Maweri; Esam Halboub; Sajna Ashraf; Ahmed Y Alqutaibi; Nashwan Mohammed Qaid; Kamila Yahya; Mohammed Nasser Alhajj Journal: BMC Oral Health Date: 2020-08-24 Impact factor: 2.757