Vitor C Chaves1,2, Laurita Boff1, Márcia Vizzotto3, Eunice Calvete4, Flávio H Reginatto2, Cláudia Mo Simões1,2. 1. Laboratório de Virologia Aplicada, Programa de Pós-Graduação em Biotecnologia e Biociências, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. 2. Laboratório de Farmacognosia, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. 3. Empresa Brasileira de Pesquisa Agropecuária de Clima Temperado (EMBRAPA), Pelotas, RS, Brazil. 4. Programa de Pós-Graduação em Agronomia, Universidade de Passo Fundo, UPF, Passo Fundo, RS, Brazil.
Abstract
BACKGROUND: Phytochemical profiles of two Brazilian native fruits - pitanga (red and purple) and araçá (yellow and red) - as well as strawberry cultivars Albion, Aromas and Camarosa, blackberry cultivar Tupy and blueberry cultivar Bluegen cultivated in Brazil were characterized for total phenolic content and total anthocyanin content by liquid chromatography coupled to a photodiode array and a quadrupole time-of-flight mass spectrometer. Radical scavenging, antiherpes and cytotoxic activities of these berry extracts were also evaluated. RESULTS: Blueberry presented the highest total anthocyanin content (1202 mg cyanidin-O-glucoside equivalents kg-1 fresh fruit), while strawberry cultivar Aromas presented the highest total phenolic content (13 550 mg gallic acid equivalents kg-1 fresh fruit). Liquid chromatographic-mass spectrometric analysis resulted in the identification of 21 anthocyanins. To the best of our knowledge this is the first report of cyanidin-O-glucoside in yellow and red Araçá fruit and the first time eight anthocyanins have been reported in pitanga fruits. DPPH and ABTS assays showed that blueberry cultivar Bluegen, blackberry cultivar Tupy and pitanga (red and purple) showed the most promising antiradical activities, respectively. No relevant cytotoxicity against three cancer cell lines or antiherpes activity was detected under the experimental conditions tested. CONCLUSION: Total anthocyanin content of all fruits had a strong positive correlation with their free radical scavenging activity, suggesting anthocyanins contribute to the antioxidant potential of these fruits.
BACKGROUND: Phytochemical profiles of two Brazilian native fruits - pitanga (red and purple) and araçá (yellow and red) - as well as strawberry cultivars Albion, Aromas and Camarosa, blackberry cultivar Tupy and blueberry cultivar Bluegen cultivated in Brazil were characterized for total phenolic content and total anthocyanin content by liquid chromatography coupled to a photodiode array and a quadrupole time-of-flight mass spectrometer. Radical scavenging, antiherpes and cytotoxic activities of these berry extracts were also evaluated. RESULTS: Blueberry presented the highest total anthocyanin content (1202 mg cyanidin-O-glucoside equivalents kg-1 fresh fruit), while strawberry cultivar Aromas presented the highest total phenolic content (13 550 mg gallic acid equivalents kg-1 fresh fruit). Liquid chromatographic-mass spectrometric analysis resulted in the identification of 21 anthocyanins. To the best of our knowledge this is the first report of cyanidin-O-glucoside in yellow and red Araçá fruit and the first time eight anthocyanins have been reported in pitanga fruits. DPPH and ABTS assays showed that blueberry cultivar Bluegen, blackberry cultivar Tupy and pitanga (red and purple) showed the most promising antiradical activities, respectively. No relevant cytotoxicity against three cancer cell lines or antiherpes activity was detected under the experimental conditions tested. CONCLUSION: Total anthocyanin content of all fruits had a strong positive correlation with their free radical scavenging activity, suggesting anthocyanins contribute to the antioxidant potential of these fruits.
Authors: Karine de Pádua Lúcio; Ana Carolina Silveira Rabelo; Carolina Morais Araújo; Geraldo Célio Brandão; Gustavo Henrique Bianco de Souza; Regislainy Gomes da Silva; Débora Maria Soares de Souza; André Talvani; Frank Silva Bezerra; Allan Jefferson Cruz Calsavara; Daniela Caldeira Costa Journal: Oxid Med Cell Longev Date: 2018-11-07 Impact factor: 6.543