| Literature DB >> 29427782 |
Ruoxin Zhang1, Jinlong Zhou2, Junrong Ren2, Siqi Sun2, Yuanyuan Di2, Hanyu Wang2, Xiaoqin An2, Kexin Zhang2, Junfeng Zhang3, Zhaoqiang Qian4, Meimei Shi4, Yanning Qiao4, Wei Ren4, Yingfang Tian5.
Abstract
Gene-environmental interaction could be the major cause of autism. The aim of the current study is to detect the effects of valproic acid on gene expression profiles and alternatively spliced genes in the prefrontal cortex in rat models of autism. Female rats received a single intraperitoneal injection of 600 mg/kg valproic acid at day 12.5 post-conception, and controls were injected with saline. Only male offspring were employed in the current study. RNA sequencing was used to investigate transcriptome in the prefrontal cortex of VPA-exposed rats. There were 3228 differently expressed genes and 637 alternative spliced genes, in VPA rats compared to controls. Pathways enrichment among the differently expressed genes and alternatively spliced genes were associated with neurological diseases and neural system development. The results implied VPA affected transcriptional and splicing events genome-wide and the transcriptional and splicing events may be associated with the autistic behaviors of VPA rats.Entities:
Keywords: Alternative splicing gene; Autism; RNA sequencing (RNA-seq); Transcriptome; Valproic acid (VPA)
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Year: 2018 PMID： 29427782 DOI： 10.1016/j.reprotox.2018.01.008
Source DB: PubMed Journal: Reprod Toxicol ISSN： 0890-6238 Impact factor: 3.143