Eun Sik Choi1, Jung Joo Yoon1, Byung Hyuk Han1, Da Hye Jeong1, Yun Jung Lee1, Dae Gill Kang2, Ho Sub Lee3. 1. College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, 460 Iksandae-ro, Jeonbuk 54538, Republic of Korea; Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University, 460 Iksandae-ro, Jeonbuk 54538, Republic of Korea. 2. College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, 460 Iksandae-ro, Jeonbuk 54538, Republic of Korea; Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University, 460 Iksandae-ro, Jeonbuk 54538, Republic of Korea. Electronic address: dgkang@wku.ac.kr. 3. College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, 460 Iksandae-ro, Jeonbuk 54538, Republic of Korea; Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University, 460 Iksandae-ro, Jeonbuk 54538, Republic of Korea. Electronic address: host@wonkwang.ac.kr.
Abstract
BACKGROUND: Ligustilide is a bioactive phthalide derivative isolated from Cnidii Rhizoma (Cnidium officinale, rhizome) and Angelicae Gigantis Radix (Angelica gigas Nakai, root) which are both medicinal herbs used to treat circulatory disorders. Vascular endothelium is a central spot in developing cardiovascular diseases and chronic vascular inflammation might result in atherosclerosis development. PURPOSE: We previously found out that a traditional herbal formula, Samul-Tang (Si-Wu-Tang, containing Cnidii Rhizoma and Angelicae Gigantis Radix), attenuated vascular inflammation in human umbilical vein endothelial cells (HUVECs). However, which compound was responsible for vascular protective action remained unclear. Here, we investigated vascular protective potential of an isolated single compound, (Z)-ligustilide. METHODS: MTT assay, western blotting, immunofluorescence, electrophoretic mobility shift assay was performed. BCECF-AM, CM-H2DCFDA, DAF-FM diacetate were used as a fluorescent indicator. RESULTS: Ligustilide suppressed HL-60 monocyte adhesion and CAMs (ICAM-1, VCAM-1, E-selectin) expression in HUVECs. Ligustilide significantly inhibited TNF-α-increased production of ROS and activated NF-κB signaling pathway. Also, ligustilide treated HUVECs exhibited significant HO-1 induction via Nrf2 nuclear translocation and endothelial NO synthesis. CONCLUSION: Present study demonstrates that ligustilde attenuates vascular inflammation and activate defense system of endothelial cell. Ligustilide is a bioactive compound which might prevent cardiovascular complications such as thrombosis or atherosclerosis.
BACKGROUND:Ligustilide is a bioactive phthalide derivative isolated from Cnidii Rhizoma (Cnidium officinale, rhizome) and Angelicae Gigantis Radix (Angelica gigas Nakai, root) which are both medicinal herbs used to treat circulatory disorders. Vascular endothelium is a central spot in developing cardiovascular diseases and chronic vascular inflammation might result in atherosclerosis development. PURPOSE: We previously found out that a traditional herbal formula, Samul-Tang (Si-Wu-Tang, containing Cnidii Rhizoma and Angelicae Gigantis Radix), attenuated vascular inflammation in human umbilical vein endothelial cells (HUVECs). However, which compound was responsible for vascular protective action remained unclear. Here, we investigated vascular protective potential of an isolated single compound, (Z)-ligustilide. METHODS:MTT assay, western blotting, immunofluorescence, electrophoretic mobility shift assay was performed. BCECF-AM, CM-H2DCFDA, DAF-FM diacetate were used as a fluorescent indicator. RESULTS:Ligustilide suppressed HL-60 monocyte adhesion and CAMs (ICAM-1, VCAM-1, E-selectin) expression in HUVECs. Ligustilide significantly inhibited TNF-α-increased production of ROS and activated NF-κB signaling pathway. Also, ligustilide treated HUVECs exhibited significant HO-1 induction via Nrf2 nuclear translocation and endothelial NO synthesis. CONCLUSION: Present study demonstrates that ligustilde attenuates vascular inflammation and activate defense system of endothelial cell. Ligustilide is a bioactive compound which might prevent cardiovascular complications such as thrombosis or atherosclerosis.
Authors: Tao Yan; Shun Guo; Tian Zhang; Zhimin Zhang; An Liu; Song Zhang; Yuan Xu; Yuhong Qi; Weihe Zhao; Qinhui Wang; Lei Shi; Linna Liu Journal: Front Pharmacol Date: 2021-04-22 Impact factor: 5.810