STUDY DESIGN: Pilot study. OBJECTIVES: Single-nucleotide polymorphisms (SNPs) in TRPA1 gene are related to the etiology of chronic pain. The study is a pilot study with the primary objective of analyzing these SNPs in Spanish patients with chronic and complete spinal cord injury (SCI) and neuropathic pain (NPP). SETTING: Asepeyo Hospital Department of Chronic and Complete SCI. METHODS: Twelve patients with chronic and complete SCI and NPP, and 12 patients with chronic and complete SCI with no pain were reviewed. International Spinal Cord Injury Pain Classification (LANSS) and visual analog score (VAS) were chosen to classify pain syndrome. SNPs were identified by melting analysis after DNA amplification with real-time fluorescence PCR. RESULTS: There were differences in rs11988795 variant: GG homozygous (p = 0.01) and G allele (p = 0.001) were more frequent in SCI patients with no pain. There were differences in rs13255063 variant: TT homozygous were prevalent (p = 0.03) in patients with NPP. CONCLUSIONS: Until now this is the first study to show a description of TRPA1 SNPs in Spanish patients with chronic and complete SCI and NPP. These results suggest that GG genotype in rs11988795 variant and G allele could be protective factors against NPP. TT genotype in rs13255063 variant could be a risk factor for NPP. Neuropathic pain after spinal cord injuries may have genetic contributions.
STUDY DESIGN: Pilot study. OBJECTIVES: Single-nucleotide polymorphisms (SNPs) in TRPA1 gene are related to the etiology of chronic pain. The study is a pilot study with the primary objective of analyzing these SNPs in Spanish patients with chronic and complete spinal cord injury (SCI) and neuropathic pain (NPP). SETTING: Asepeyo Hospital Department of Chronic and Complete SCI. METHODS: Twelve patients with chronic and complete SCI and NPP, and 12 patients with chronic and complete SCI with no pain were reviewed. International Spinal Cord Injury Pain Classification (LANSS) and visual analog score (VAS) were chosen to classify pain syndrome. SNPs were identified by melting analysis after DNA amplification with real-time fluorescence PCR. RESULTS: There were differences in rs11988795 variant: GG homozygous (p = 0.01) and G allele (p = 0.001) were more frequent in SCI patients with no pain. There were differences in rs13255063 variant: TT homozygous were prevalent (p = 0.03) in patients with NPP. CONCLUSIONS: Until now this is the first study to show a description of TRPA1 SNPs in Spanish patients with chronic and complete SCI and NPP. These results suggest that GG genotype in rs11988795 variant and G allele could be protective factors against NPP. TT genotype in rs13255063 variant could be a risk factor for NPP. Neuropathic pain after spinal cord injuries may have genetic contributions.
Authors: David A Andersson; Clive Gentry; Lisa Alenmyr; Dan Killander; Simon E Lewis; Anders Andersson; Bernard Bucher; Jean-Luc Galzi; Olov Sterner; Stuart Bevan; Edward D Högestätt; Peter M Zygmunt Journal: Nat Commun Date: 2011-11-22 Impact factor: 14.919
Authors: Donato del Camino; Sarah Murphy; Melissa Heiry; Lee B Barrett; Taryn J Earley; Colby A Cook; Matt J Petrus; Michael Zhao; Marc D'Amours; Nate Deering; Gary J Brenner; Michael Costigan; Neil J Hayward; Jayhong A Chong; Christopher M Fanger; Clifford J Woolf; Ardem Patapoutian; Magdalene M Moran Journal: J Neurosci Date: 2010-11-10 Impact factor: 6.167
Authors: Asad E Patanwala; Charles Norwood; Heidi Steiner; Daniel Morrison; May Li; Keith Walsh; Marina Martinez; Sarah E Baker; Eric M Snyder; Jason H Karnes Journal: Clin Transl Sci Date: 2018-12-31 Impact factor: 4.689