Raymond S W Tsang1, Tauqeer Ahmad2, Shaun Tyler2, Brigitte Lefebvre3, Shelley L Deeks4, Rodica Gilca5, Linda Hoang6, Gregory Tyrrell7, Paul Van Caeseele8, Gary Van Domselaar9, Frances B Jamieson10. 1. Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada. Electronic address: raymond.tsang@canada.ca. 2. Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada. 3. Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, Ste-Anne-Bellevue, Québec, Canada. 4. Public Health Ontario, Toronto, Ontario, Canada; Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario, Canada. 5. Institut National de Santé Publique du Québec, Centre de Recherche du CHU-Université Laval, Québec, Canada; Department de Medecine Sociale et Preventive de l'Université Laval, Québec, Canada. 6. BC Public Health Microbiology and Reference Laboratory, Vancouver, British Columbia, Canada. 7. Provincial Laboratory for Public Health, Edmonton, Alberta, Canada. 8. Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada. 9. Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada; Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada. 10. Public Health Ontario, Toronto, Ontario, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
Abstract
OBJECTIVES: This study was performed to analyze the Canadian invasive serogroup W Neisseria meningitidis (MenW) sequence type 11 (ST-11) clonal complex (CC) isolates by whole genome typing and to compare Canadian isolates with similar isolates from elsewhere. METHODS: Whole genome typing of 30 MenW ST-11 CC, 20 meningococcal group C (MenC) ST-11 CC, and 31 MenW ST-22 CC isolates was performed on the Bacterial Isolate Genome Sequence database platform. Canadian MenW ST-11 CC isolates were compared with the 2000 MenW Hajj outbreak strain, as well as with MenW ST-11 CC from other countries. RESULTS: Whole genome typing showed that the Canadian MenW ST-11 CC isolates were distinct from the traditional MenW ST-22 CC; they were not capsule-switched contemporary MenC strains that incorporated MenW capsules. While some recent MenW disease cases in Canada were caused by MenW ST-11 CC isolates showing relatedness to the 2000 MenW Hajj strain, many were non-Hajj isolates similar to current MenW ST-11 isolates found globally. Geographical and temporal variations in genotypes and surface protein antigen genes were found among the MenW ST-11 CC isolates. CONCLUSIONS: The current MenW ST-11 isolates did not arise by capsule switching from contemporary MenC ST-11 isolates. Both the Hajj-related and non-Hajj MenW ST-11 CC strains were associated with invasive meningococcal disease in Canada.
OBJECTIVES: This study was performed to analyze the Canadian invasive serogroup W Neisseria meningitidis (MenW) sequence type 11 (ST-11) clonal complex (CC) isolates by whole genome typing and to compare Canadian isolates with similar isolates from elsewhere. METHODS: Whole genome typing of 30 MenW ST-11 CC, 20 meningococcal group C (MenC) ST-11 CC, and 31 MenW ST-22 CC isolates was performed on the Bacterial Isolate Genome Sequence database platform. Canadian MenW ST-11 CC isolates were compared with the 2000 MenW Hajj outbreak strain, as well as with MenW ST-11 CC from other countries. RESULTS: Whole genome typing showed that the Canadian MenW ST-11 CC isolates were distinct from the traditional MenW ST-22 CC; they were not capsule-switched contemporary MenC strains that incorporated MenW capsules. While some recent MenW disease cases in Canada were caused by MenW ST-11 CC isolates showing relatedness to the 2000 MenW Hajj strain, many were non-Hajj isolates similar to current MenW ST-11 isolates found globally. Geographical and temporal variations in genotypes and surface protein antigen genes were found among the MenW ST-11 CC isolates. CONCLUSIONS: The current MenW ST-11 isolates did not arise by capsule switching from contemporary MenC ST-11 isolates. Both the Hajj-related and non-Hajj MenW ST-11 CC strains were associated with invasive meningococcal disease in Canada.
Authors: R S Tsang; L Hoang; G J Tyrrell; J Minion; P Van Caeseele; J V Kus; B Lefebvre; D Haldane; R Garceau; G German; G Zahariadis; B Hanley Journal: Can Commun Dis Rep Date: 2019-06-06
Authors: Jonathan Lawler; Jay Lucidarme; Sydel Parikh; Lorna Smith; Helen Campbell; Ray Borrow; Steve Gray; Kirsty Foster; Shamez Ladhani Journal: Euro Surveill Date: 2019-06
Authors: Manuel Krone; Steve Gray; Raquel Abad; Anna Skoczyńska; Paola Stefanelli; Arie van der Ende; Georgina Tzanakaki; Paula Mölling; Maria João Simões; Pavla Křížová; Stéphane Emonet; Dominique A Caugant; Maija Toropainen; Julio Vazquez; Izabela Waśko; Mirjam J Knol; Susanne Jacobsson; Célia Rodrigues Bettencourt; Martin Musilek; Rita Born; Ulrich Vogel; Ray Borrow Journal: Euro Surveill Date: 2019-04