| Literature DB >> 29414603 |
Ruoji Zhou1, An Xu2, Donghui Wang3, Dandan Zhu2, Helen Mata4, Zijun Huo5, Jian Tu6, Mo Liu2, Alaa M T Mohamed2, Brittany E Jewell1, Julian Gingold7, Weiya Xia8, Pulivarthi H Rao4, Mien-Chie Hung9, Ruiying Zhao10, Dung-Fang Lee11.
Abstract
The tumor suppressor gene TP53 is the most frequently mutated gene in human cancers. Many hot-spot mutations of TP53 confer novel functions not found in wild-type p53 and contribute to tumor development and progression. We report on the generation of a H1 human embryonic stem cell line carrying a homozygous TP53 R282W mutation using TALEN-mediated genome editing. The generated cell line demonstrates normal karyotype, maintains a pluripotent state, and is capable of generating a teratoma in vivo containing tissues from all three germ layers.Entities:
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Year: 2018 PMID： 29414603 DOI： 10.1016/j.scr.2018.01.035
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020