Gerardo Maria de Araújo Filho1, Denise Poltronieri Martins2, Angélica Marta Lopes2, Beatriz de Jesus Brait2, Ana Eliza Romano Furlan3, Camila Ive Ferreira Oliveira2, Lucia Helena Neves Marques4, Dorotéia Rossi Silva Souza2, Eduardo Alves de Almeida5. 1. Department of Psychiatry and Medical Psychology, Faculdade de Medicina de São José do Rio Preto (FAMERP), São Paulo, Brazil. Electronic address: gerardo.filho@famerp.br. 2. Department of Biochemistry and Molecular Biology-NPBIM, Faculdade de Medicina de São José do Rio Preto (FAMERP), São Paulo, Brazil. 3. Department of Psychiatry and Medical Psychology, Faculdade de Medicina de São José do Rio Preto (FAMERP), São Paulo, Brazil. 4. Department of Neurological Sciences, Faculdade de Medicina de São José do Rio Preto (FAMERP), São Paulo, Brazil. 5. Department of Chemistry and Environmental Sciences - IBILCE, Universidade Estadual Paulista (UNESP), São Paulo, Brazil.
Abstract
OBJECTIVE: The objective was to evaluate the genetic and biochemical profiles associated with oxidative stress (OS) in patients with temporal lobe epilepsy with mesial temporal sclerosis (TLE-MTS) and a healthy control group, and also to verify the possible existence of association between OS markers and psychiatric disorders (PD) in group with TLE-MTS. METHODS: Forty-six patients with refractory TLE-MTS and 112 healthy controls were included. Psychiatric evaluation occurred through Diagnostical and Statistical Manual of Mental Disorders (DSM-5) criteria. A peripheral blood sample was collected for analysis of glutathione S-transferase (GST) T1/M1 polymorphisms and serum levels of malondialdehyde (MDA) and antioxidant capacity equivalent to the trolox (TEAC), serum markers of OS. Student's t-test, Fisher's exact test, Chi-square test, and Analysis of Variance (ANOVA) were used, with a significance level of P<0.05. RESULTS: The PD were observed in 27 patients of the group with TLE-MTS (58.6%); major depressive disorder (MDD) was the most frequent. Serum levels of MDA (P<0.0001) and TEAC (P<0.0001) were higher in group with TLE-MTS. When patients with MDD were compared with patients without PD, significant differences were observed between MDA (P=0.002) and TEAC (P=0.003) serum levels. Patients with TLE-MTS and MDD presented higher levels when compared with patients with TLE-MTS without PD and with another PD except MDD. CONCLUSIONS: The present study observed significantly higher serum levels of MDA and of TEAC in patients with refractory TLE-MTS in comparison with the control group. The MDD was observed as an important issue associated with higher OS levels in refractory TLE-MTS. Further studies are needed to investigate the association of OS, TLE-MTS, and PD.
OBJECTIVE: The objective was to evaluate the genetic and biochemical profiles associated with oxidative stress (OS) in patients with temporal lobe epilepsy with mesial temporal sclerosis (TLE-MTS) and a healthy control group, and also to verify the possible existence of association between OS markers and psychiatric disorders (PD) in group with TLE-MTS. METHODS: Forty-six patients with refractory TLE-MTS and 112 healthy controls were included. Psychiatric evaluation occurred through Diagnostical and Statistical Manual of Mental Disorders (DSM-5) criteria. A peripheral blood sample was collected for analysis of glutathione S-transferase (GST) T1/M1 polymorphisms and serum levels of malondialdehyde (MDA) and antioxidant capacity equivalent to the trolox (TEAC), serum markers of OS. Student's t-test, Fisher's exact test, Chi-square test, and Analysis of Variance (ANOVA) were used, with a significance level of P<0.05. RESULTS: The PD were observed in 27 patients of the group with TLE-MTS (58.6%); major depressive disorder (MDD) was the most frequent. Serum levels of MDA (P<0.0001) and TEAC (P<0.0001) were higher in group with TLE-MTS. When patients with MDD were compared with patients without PD, significant differences were observed between MDA (P=0.002) and TEAC (P=0.003) serum levels. Patients with TLE-MTS and MDD presented higher levels when compared with patients with TLE-MTS without PD and with another PD except MDD. CONCLUSIONS: The present study observed significantly higher serum levels of MDA and of TEAC in patients with refractory TLE-MTS in comparison with the control group. The MDD was observed as an important issue associated with higher OS levels in refractory TLE-MTS. Further studies are needed to investigate the association of OS, TLE-MTS, and PD.
Authors: Alicia Gonzalez-Martinez; Álvaro Planchuelo-Gómez; Alba Vieira Campos; Francisco Martínez-Dubarbie; José Vivancos; María De Toledo-Heras Journal: Neurol Sci Date: 2021-09-15 Impact factor: 3.830
Authors: Patrick Buosi; Fábio Aparecido Borghi; Angélica Marta Lopes; Isabela da Silva Facincani; Rafael Fernandes-Ferreira; Camila Ive Ferreira Oliveira-Brancati; Tayanne Silva do Carmo; Dorotéia Rossi Silva Souza; Danilo Grünig Humberto da Silva; Eduardo Alves de Almeida; Gerardo Maria de Araújo Filho Journal: Trends Psychiatry Psychother Date: 2021-01-01