Literature DB >> 2941014

Agonist-induced calcium transients in cultured smooth muscle cells: measurements with fura-2 loaded monolayers.

E E Reynolds, G R Dubyak.   

Abstract

Elevation of cytosolic Ca2+ in response to depolarization and various receptor agonists was measured in several types of cultured smooth muscle cells (DDT1, A10, rabbit aorta) loaded with the either quin-2 or fura-2, and assayed either in suspension or in monolayer cultures attached to plastic cover slips. Agonists (norepinephrine, vasopressin) induced both the release of intracellular Ca2+ and the influx of extracellular Ca2+. Agonist-induced Ca2+ influx was not blocked by dihydropyridines, and depolarization did not induce Ca2+ influx. However, in fura-2 loaded monolayers of PC12 cells, depolarization did induce dihydropyridine-sensitive Ca2+ influx. Thus cultured smooth muscle cells appear to express receptor-operated Ca2+ channels, but not functional voltage-operated Ca2+ channels.

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Year:  1986        PMID： 2941014     DOI： 10.1016/0006-291x(86)90421-3

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

1.  Alkalinization stimulates the purified plasma-membrane Ca2+ pump by increasing its Ca2+ affinity.

Authors:  L Missiaen; G Droogmans; H De Smedt; F Wuytack; L Raeymaekers; R Casteels
Journal:  Biochem J       Date:  1989-08-15       Impact factor: 3.857

2.  Neurotransmitter release from bradykinin-stimulated PC12 cells. Stimulation of cytosolic calcium and neurotransmitter release.

Authors:  K C Appell; D S Barefoot
Journal:  Biochem J       Date:  1989-10-01       Impact factor: 3.857

3.  The oligosaccharide component of alpha 1-adrenergic receptors from BC3H1 and DDT1 muscle cells. Studies with glycosidases and photoaffinity labelling of intact cells.

Authors:  B I Terman; J F Reece; R D Brown; P A Insel
Journal:  Biochem J       Date:  1988-07-15       Impact factor: 3.857

4.  Temporal changes in the calcium-dependence of the histamine H1-receptor-stimulation of cyclic AMP accumulation in guinea-pig cerebral cortex.

Authors:  J Donaldson; A M Brown; S J Hill
Journal:  Br J Pharmacol       Date:  1989-12       Impact factor: 8.739

5.  Calcium release from separate receptor-specific intracellular stores induced by histamine and ATP in a hamster cell line.

Authors:  A Den Hertog; B Hoiting; A Molleman; J Van den Akker; M Duin; A Nelemans
Journal:  J Physiol       Date:  1992-08       Impact factor: 5.182

6.  A novel tumour promoter, thapsigargin, transiently increases cytoplasmic free Ca2+ without generation of inositol phosphates in NG115-401L neuronal cells.

Authors:  T R Jackson; S I Patterson; O Thastrup; M R Hanley
Journal:  Biochem J       Date:  1988-07-01       Impact factor: 3.857

7.  Calcium and cyclic adenosine monophosphate as second messengers for vasopressin in the rat inner medullary collecting duct.

Authors:  R A Star; H Nonoguchi; R Balaban; M A Knepper
Journal:  J Clin Invest       Date:  1988-06       Impact factor: 14.808

8.  Measurement of cytoplasmic free Ca2+ concentration in rabbit aorta using the photoprotein, aequorin. Effect of atrial natriuretic peptide on agonist-induced Ca2+ signal generation.

Authors:  Y Takuwa; H Rasmussen
Journal:  J Clin Invest       Date:  1987-07       Impact factor: 14.808

9.  Receptor coupled events in bradykinin action: rapid production of inositol phosphates and regulation of cytosolic free Ca2+ in a neural cell line.

Authors:  T R Jackson; T J Hallam; C P Downes; M R Hanley
Journal:  EMBO J       Date:  1987-01       Impact factor: 11.598
  9 in total

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