K R Nirupama1, Vinodh Kumar O R2, B S Pruthvishree1, D K Sinha1, M Senthil Murugan1, Narayanan Krishnaswamy3, B R Singh1. 1. Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Bareilly, India. 2. Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Bareilly, India. Electronic address: vinodhkumar.rajendran@gmail.com. 3. Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Bareilly, India.
Abstract
OBJECTIVES: The aim of this study was to characterise carbapenemase-, extended-spectrum β-lactamase (ESBL)- and Shiga toxin-producing Escherichia coli isolated from farm piglets in India. METHODS: Faecal samples (n=741) from 10 organised pig farms, including non-diarrhoeic (n=546) and diarrhoeic (n=195) piglets, were processed for isolation of carbapenem-resistant and ESBL-producing E. coli. RESULTS: A total of 27 and 243 isolates were phenotypically confirmed as carbapenem-resistant and ESBL-producers, respectively. The meropenem minimum inhibitory concentration (MIC) of carbapenem-resistant isolates ranged from 8-128μg/mL. On genotypic screening of the 27 carbapenem-resistant isolates, 3 isolates were positive for the blaOXA-48 carbapenemase gene; no other carbapenemase genes were detected. The 243 ESBL-producing isolates were positive for blaCTX-M-1 (n=135), qnrA (n=92), qnrB (n=112), qnrS (n=49), tetA (n=42), tetB (n=45) and sul1 (n=43). The Shiga toxin virulence markers stx1 and stx2 were detected in 41 and 38 of the 243 phenotypic ESBL-producing isolates, respectively. Multilocus sequence typing (MLST) of blaOXA-48-positive E. coli isolates showed ST10- and ST5053-like sequence types. CONCLUSION: This is the first report on the presence of blaOXA-48-carrying E. coli in piglets in India, which pose a potential risk to public health.
OBJECTIVES: The aim of this study was to characterise carbapenemase-, extended-spectrum β-lactamase (ESBL)- and Shiga toxin-producing Escherichia coli isolated from farm piglets in India. METHODS: Faecal samples (n=741) from 10 organised pig farms, including non-diarrhoeic (n=546) and diarrhoeic (n=195) piglets, were processed for isolation of carbapenem-resistant and ESBL-producing E. coli. RESULTS: A total of 27 and 243 isolates were phenotypically confirmed as carbapenem-resistant and ESBL-producers, respectively. The meropenem minimum inhibitory concentration (MIC) of carbapenem-resistant isolates ranged from 8-128μg/mL. On genotypic screening of the 27 carbapenem-resistant isolates, 3 isolates were positive for the blaOXA-48 carbapenemase gene; no other carbapenemase genes were detected. The 243 ESBL-producing isolates were positive for blaCTX-M-1 (n=135), qnrA (n=92), qnrB (n=112), qnrS (n=49), tetA (n=42), tetB (n=45) and sul1 (n=43). The Shiga toxin virulence markers stx1 and stx2 were detected in 41 and 38 of the 243 phenotypic ESBL-producing isolates, respectively. Multilocus sequence typing (MLST) of blaOXA-48-positive E. coli isolates showed ST10- and ST5053-like sequence types. CONCLUSION: This is the first report on the presence of blaOXA-48-carrying E. coli in piglets in India, which pose a potential risk to public health.
Authors: O R VinodhKumar; S Sircar; B S Pruthvishree; K R Nirupama; B R Singh; D K Sinha; Ramkumar Rupner; A Karthikeyan; R Karthikeyan; Z B Dubal; Y S Malik Journal: Trop Anim Health Prod Date: 2019-07-17 Impact factor: 1.559
Authors: Marília Miotto; Sylvia A Ossai; Joan E Meredith; Clarissa Barretta; Airton Kist; Elane S Prudencio; Cleide R W Vieira; Salina Parveen Journal: Microbiologyopen Date: 2018-10-11 Impact factor: 3.139
Authors: O R VinodhKumar; B R Singh; D K Sinha; B S Pruthvishree; Shika Tamta; Z B Dubal; R Karthikeyan; Ramkumar N Rupner; Y S Malik Journal: Epidemiol Infect Date: 2019-01 Impact factor: 2.451
Authors: M Senthil Murugan; D K Sinha; O R Vinodh Kumar; Ajay Kumar Yadav; B S Pruthvishree; Prasanna Vadhana; K R Nirupama; Monika Bhardwaj; B R Singh Journal: Epidemiol Infect Date: 2019-01 Impact factor: 2.451