Induction of cellular DNA synthesis by purified adenovirus E1A proteins.

The purified Escherichia coli-expressed products of the human subgroup-C adenovirus E1A 13 S and 12 S mRNAs are shown to induce cellular DNA synthesis when introduced by microinjection into quiescent, G0-arrested mammalian cells from immortalized cell lines. The E1A proteins stimulated cellular DNA synthesis in mouse Swiss 3T3 cells, when microinjected either individually or in combination. A truncated E1A protein, in which 169 carboxyl terminal residues of the 289-amino acid E1A 13 S mRNA product are deleted, was unable to induce cellular DNA synthesis in these cells. Our results provide evidence that E1A proteins can function, independent of other viral functions, in the stimulation of cellular DNA synthesis in certain cell types. The present results are consistent with the E1A gene products acting to modulate the expression of the cellular genes which control cell cycle progression into S phase.