Lifeng Cui1, Chang Shu2, Zitao Liu3, Weihua Tong4, Miao Cui5, Chengguo Wei6, Jian Jenny Tang7, Xiufen Liu8, Hujing Hai9, Jing Jiang10, Jin He2, David Y Zhang5, Fei Ye5, Yulin Li11. 1. Department of Pathology, College of Basic Medical Sciences of Jilin University, Changchun, Jilin 130021, China. 2. Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun, Jilin 130021, China. 3. New Hope Fertility Center, New York, NY10019, USA. 4. Department of Gastrointestinal Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, China. 5. Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. 6. Department of Medicine Bioinformatics Core, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. 7. Department of Obstetrics, Gynecology and Reproductive Science, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. 8. Department of Ophthalmology, The First Hospital of Jilin University, Changchun, Jilin 130021, China. 9. Department of Urology, The First Hospital of Jilin University, Changchun, Jilin 130021, China. 10. Division of Clinical Epidemiology, The First Hospital of Jilin University, Changchun, Jilin, China. 11. Department of Pathology, College of Basic Medical Sciences of Jilin University, Changchun, Jilin 130021, China. Electronic address: lylchina78@yahoo.com.
Abstract
BACKGROUND: Preeclampsia is a multi-system disorder in pregnancy which has no effective treatment. The diagnosis of preeclampsia is based on clinical presentation and routine laboratory tests. OBJECTIVE: This study aimed at identifying serum protein markers for diagnosis of preeclampsia and predicting its severe features. STUDY DESIGN: In total, 172 pregnant women were enrolled in this study including 110 subjects with preeclampsia and 62 normotensive subjects. Eleven serum proteins (VEGF, sFlt-1, sEndoglin, PlGF, sEGFR, prolactin, PTX3, PAI-1, NGAL, IL-27, COX-2) were assessed using Luminex multiplex immunoassay and ELISA. RESULTS: The levels of seven proteins (sFlt-1, VEGF, sEndoglin, sEGFR, PlGF, NGAL, COX-2) correlated with preeclampsia, and 4 proteins (VEGF, sEndoglin, PlGF, sEGFR) were identified as independent factors associated with preeclampsia. The levels of three proteins (sEndoglin, PTX3, sFlt-1) correlated with severe features of preeclampsia, and three variables (serum creatinine, platelet count and sEndoglin) were identified as independent factors in predicting severe features of preeclampsia. CONCLUSIONS: A combination of serum protein markers (VEGF, sEndoglin, PlGF, sEGFR) and clinical variables (serum creatinine, platelet count and sEndoglin) could be used as analytical tool in diagnosis of preeclampsia and its severe features, respectively. Serum sEGFR, a novel biomarker in preeclampsia, may be involved in the pathogenesis of preeclampsia.
BACKGROUND: Preeclampsia is a multi-system disorder in pregnancy which has no effective treatment. The diagnosis of preeclampsia is based on clinical presentation and routine laboratory tests. OBJECTIVE: This study aimed at identifying serum protein markers for diagnosis of preeclampsia and predicting its severe features. STUDY DESIGN: In total, 172 pregnant women were enrolled in this study including 110 subjects with preeclampsia and 62 normotensive subjects. Eleven serum proteins (VEGF, sFlt-1, sEndoglin, PlGF, sEGFR, prolactin, PTX3, PAI-1, NGAL, IL-27, COX-2) were assessed using Luminex multiplex immunoassay and ELISA. RESULTS: The levels of seven proteins (sFlt-1, VEGF, sEndoglin, sEGFR, PlGF, NGAL, COX-2) correlated with preeclampsia, and 4 proteins (VEGF, sEndoglin, PlGF, sEGFR) were identified as independent factors associated with preeclampsia. The levels of three proteins (sEndoglin, PTX3, sFlt-1) correlated with severe features of preeclampsia, and three variables (serum creatinine, platelet count and sEndoglin) were identified as independent factors in predicting severe features of preeclampsia. CONCLUSIONS: A combination of serum protein markers (VEGF, sEndoglin, PlGF, sEGFR) and clinical variables (serum creatinine, platelet count and sEndoglin) could be used as analytical tool in diagnosis of preeclampsia and its severe features, respectively. Serum sEGFR, a novel biomarker in preeclampsia, may be involved in the pathogenesis of preeclampsia.
Authors: Alejandra Abascal-Saiz; Marta Duque-Alcorta; Victoria Fioravantti; Eugenia Antolín; Eva Fuente-Luelmo; María Haro; María P Ramos-Álvarez; Germán Perdomo; José L Bartha Journal: Nutrients Date: 2022-05-23 Impact factor: 6.706