Anthony Almudevar1, Michael E Pichichero2. 1. Department of Biostatistics and Computational Biology, University of Rochester Medical Center, Rochester, NY, USA. 2. Center for Infectious Disease and Immunology, Rochester General Hospital Research Institute, Rochester, NY, USA. Electronic address: michael.pichichero@rochesterregional.org.
Abstract
BACKGROUND: Haemophilus influenzae (Hi) causes respiratory infections and pathogenesis of this microbe begins in the human nasopharynx (NP). The objective of this study was to assess the correlation of NP colonization-induced serum antibody levels to Hi protein D with risk of acute otitis media (AOM) in children <2 yr. METHODS: 455 sera from 213 children (age 6-24 months old) were collected when they were colonized with Hi and when the children developed AOM. Presence of Hi during AOM was confirmed by culture of middle ear fluid. Quantitative ELISA was used to determine serum IgG against protein D antigen. RESULTS: Asymptomatic Hi NP colonization reduced the risk of future AOM infections. Higher serum IgG titers against Hi protein D were correlated with reduced future AOM risk. CONCLUSION: Colonization by Hi reduces future AOM risk. Higher antibody levels against protein D correlates with lower risk of AOM caused by Hi.
BACKGROUND:Haemophilus influenzae (Hi) causes respiratory infections and pathogenesis of this microbe begins in the human nasopharynx (NP). The objective of this study was to assess the correlation of NP colonization-induced serum antibody levels to Hiprotein D with risk of acute otitis media (AOM) in children <2 yr. METHODS: 455 sera from 213 children (age 6-24 months old) were collected when they were colonized with Hi and when the children developed AOM. Presence of Hi during AOM was confirmed by culture of middle ear fluid. Quantitative ELISA was used to determine serum IgG against protein D antigen. RESULTS: Asymptomatic Hi NP colonization reduced the risk of future AOM infections. Higher serum IgG titers against Hiprotein D were correlated with reduced future AOM risk. CONCLUSION: Colonization by Hi reduces future AOM risk. Higher antibody levels against protein D correlates with lower risk of AOM caused by Hi.
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