| Literature DB >> 29392674 |
Xuefei Cui1,2, Jing Zheng1,2, Jinxin Zheng1,2, Qingqiu Gong3,4.
Abstract
As a major intracellular degradation pathway, autophagy contributes to nutrient recycling and is indispensable during plant senescence. Here we describe methods used for investigating the autophagic process during leaf senescence. These include transcript analysis of core machinery autophagy genes, immunoblotting of ATG8, and microscopic observation of autophagosome formation.Entities:
Keywords: ATG8; Autophagy; Confocal laser scanning microscopy; Immunoblots; Leaf senescence; Transcript analysis
Mesh：
Year: 2018 PMID： 29392674 DOI： 10.1007/978-1-4939-7672-0_23
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745