Literature DB >> 29392166

Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization.

Ting-Hai Xu1,2,3, Yan Yan1,2,3, Kaleeckal G Harikumar4, Laurence J Miller4, Karsten Melcher3, H Eric Xu1,3.   

Abstract

Pulldown assay is a conventional method to determine protein-protein interactions in vitro. Expressing a protein of interest with two different tags allows testing whether both versions can be captured via one of the two tags as homooligomeric complex. This protocol is based on streptavidin bead capture of a biotinylated protein and co-associated Flag-tagged protein using Streptavidin MagBeads.

Entities:  

Keywords:  Biotinylated protein; Homooligomeric complex; Protein-protein interactions; Pulldown assay; Streptavidin bead

Year:  2017        PMID： 29392166      PMCID： PMC5790316          DOI： 10.21769/BioProtoc.2901

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  2 in total

1.  Dimerization of the transmembrane domain of amyloid precursor protein is determined by residues around the γ-secretase cleavage sites.

Authors:  Yan Yan; Ting-Hai Xu; Kaleeckal G Harikumar; Laurence J Miller; Karsten Melcher; H Eric Xu
Journal:  J Biol Chem       Date:  2017-08-08       Impact factor: 5.157

2.  Alzheimer's disease-associated mutations increase amyloid precursor protein resistance to γ-secretase cleavage and the Aβ42/Aβ40 ratio.

Authors:  Ting-Hai Xu; Yan Yan; Yanyong Kang; Yi Jiang; Karsten Melcher; H Eric Xu
Journal:  Cell Discov       Date:  2016-08-23       Impact factor: 10.849
  2 in total

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