Ufuk Düzenli1, Yüksel Olgun2, Safiye Aktaş3, Ayça Pamukoğlu3, Zekiye Altun3. 1. Clinic of Otorhinolaryngology, İzmir Bozyaka Training and Research Hospital, İzmir, Turkey. 2. Department of Otorhinolaryngology, Dokuz Eylül University School of Medicine, İzmir, Turkey. 3. Department of Basic Oncology, Institute of Oncology, Dokuz Eylül University School of Medicine, İzmir, Turkey.
Abstract
OBJECTIVE: Cisplatin is a widely used agent for the treatment of adult and childhood malignancies. Side effects such as nephrotoxicity, neurotoxicity, and ototoxicity lead to dose limitations. Ecklonia cava polyphenol extract (ECP) is a molecule obtained from algae that live in seawater in the Far East. ECP has recently been shown to have protective effects against oxidative stress. The aim of this study was to evaluate the possible protective effects of ECP on cisplatin ototoxicity. METHODS: In this study, we investigated the protective effects of ECP against cisplatin-induced cell death in mouse-derived House Ear Institute Organ of Corti (HEI-OC1) cochlear cells. Cisplatin (100 μM) and 1, 10, and 25 μM doses of ECP were administered to the cells, and the protective effects of ECP at 24 and 72 hours were investigated. Cell viability was evaluated by the WST-1 (water soluble tetrazolium salt). RESULTS: Cisplatin (100 μM) reduced cell viability in both the 24th and 72nd hour evaluation. Although the 25 μM dose of ECP showed otoprotective effects in the 24th hour, in the 72nd hour this effect disappeared. Other doses of ECP showed no otoprotective effects in the 24th and 72nd hours. CONCLUSION: Although ECP showed some protective effects in the 24th hour against cisplatin ototoxicity, these effects disappeared by the 72nd hour. Further studies using recurrent and higher doses of ECP are required.
OBJECTIVE: Cisplatin is a widely used agent for the treatment of adult and childhood malignancies. Side effects such as nephrotoxicity, neurotoxicity, and ototoxicity lead to dose limitations. Ecklonia cava polyphenol extract (ECP) is a molecule obtained from algae that live in seawater in the Far East. ECP has recently been shown to have protective effects against oxidative stress. The aim of this study was to evaluate the possible protective effects of ECP on cisplatin ototoxicity. METHODS: In this study, we investigated the protective effects of ECP against cisplatin-induced cell death in mouse-derived House Ear Institute Organ of Corti (HEI-OC1) cochlear cells. Cisplatin (100 μM) and 1, 10, and 25 μM doses of ECP were administered to the cells, and the protective effects of ECP at 24 and 72 hours were investigated. Cell viability was evaluated by the WST-1 (water soluble tetrazolium salt). RESULTS: Cisplatin (100 μM) reduced cell viability in both the 24th and 72nd hour evaluation. Although the 25 μM dose of ECP showed otoprotective effects in the 24th hour, in the 72nd hour this effect disappeared. Other doses of ECP showed no otoprotective effects in the 24th and 72nd hours. CONCLUSION: Although ECP showed some protective effects in the 24th hour against cisplatin ototoxicity, these effects disappeared by the 72nd hour. Further studies using recurrent and higher doses of ECP are required.
Authors: Mun Young Chang; Seon-Hee Byon; Hyeon-Cheol Shin; Song Ee Han; Ju Yeon Kim; Jang Yul Byun; Jong Dae Lee; Moo Kyun Park Journal: Int J Pediatr Otorhinolaryngol Date: 2016-01-25 Impact factor: 1.675
Authors: Z Altun; Y Olgun; P Ercetin; S Aktas; G Kirkim; B Serbetcioglu; N Olgun; E A Guneri Journal: Cell Prolif Date: 2013-11-29 Impact factor: 6.831