Literature DB >> 29391178

Triplex real-time PCR method for the qualitative detection of European and American foulbrood in honeybee.

Benjamin Dainat1, Daniela Grossar2, Brigitte Ecoffey3, Christoph Haldemann3.   

Abstract

The bacteria Melissococcus plutonius and Paenibacillus larvae, causative agents of respectively European and American foulbrood, damage honeybee health worldwide. Here, we present a specific and sensitive qualitative triplex real-time PCR method to detect simultaneously those microbial agents and a honeybee gene, validated through a study involving 7 laboratories through Europe.
Copyright © 2018 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Apis mellifera; Diagnosis; Melissococcus plutonius; Multiplex real-time PCR; Paenibacillus larvae

Mesh：

Substances：

Year:  2018        PMID： 29391178     DOI： 10.1016/j.mimet.2018.01.018

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  5 in total

Review 1.  Molecular Detection and Differentiation of Arthropod, Fungal, Protozoan, Bacterial and Viral Pathogens of Honeybees.

Authors:  Lucas Lannutti; Fernanda Noemi Gonzales; Maria José Dus Santos; Mónica Florin-Christensen; Leonhard Schnittger
Journal:  Vet Sci       Date:  2022-05-02

2.  Melissococcus plutonius Can Be Effectively and Economically Detected Using Hive Debris and Conventional PCR.

Authors:  Jana Biová; Jean-Daniel Charrière; Silvie Dostálková; Mária Škrabišová; Marek Petřivalský; Jaroslav Bzdil; Jiří Danihlík
Journal:  Insects       Date:  2021-02-09       Impact factor: 2.769

3.  A novel multiplex PCR assay to detect and distinguish between different types of Paenibacillus larvae and Melissococcus plutonius, and a survey of foulbrood pathogen contamination in Japanese honey.

Authors:  Mariko Okamoto; Hirotaka Furuya; Ikuko Sugimoto; Masahiro Kusumoto; Daisuke Takamatsu
Journal:  J Vet Med Sci       Date:  2022-01-26       Impact factor: 1.267

4.  Lack of evidence for trans-generational immune priming against the honey bee pathogen Melissococcus plutonius.

Authors:  Florine Ory; Vincent Duchemin; Verena Kilchenmann; Jean-Daniel Charrière; Benjamin Dainat; Vincent Dietemann
Journal:  PLoS One       Date:  2022-05-09       Impact factor: 3.752

5.  Evaluation of Quantitative PCR (qPCR) Paenibacillus larvae Targeted Assays and Definition of Optimal Conditions for Its Detection/Quantification in Honey and Hive Debris.

Authors:  Franca Rossi; Carmela Amadoro; Addolorato Ruberto; Luciano Ricchiuti
Journal:  Insects       Date:  2018-11-16       Impact factor: 2.769
  5 in total

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