Literature DB >> 2938521

Sequential detection of antigens in Western blots with differently colored products.

N Theisen, E S Lohoff, K von Figura, A Hasilik.   

Abstract

A reliable sequential application of peroxidase and alkaline phosphatase-conjugated second antibodies to detection of two different antigens in Western blots is described. Deposits of differently colored products formed allow identification of several antigens on a single blot.

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Year:  1986        PMID： 2938521     DOI： 10.1016/0003-2697(86)90399-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Proteolytic processing and glycosylation of cathepsin B. The role of the primary structure of the latent precursor and of the carbohydrate moiety for cell-type-specific molecular forms of the enzyme.

Authors:  L Mach; K Stüwe; A Hagen; C Ballaun; J Glössl
Journal:  Biochem J       Date:  1992-03-01       Impact factor: 3.857

2.  Evidence that the middle T antigen of polyomavirus interacts with the membrane skeleton.

Authors:  D W Andrews; J Gupta; G Abisdris
Journal:  Mol Cell Biol       Date:  1993-08       Impact factor: 4.272

3.  The antigenicity of the carbohydrate moiety of an insect glycoprotein, honey-bee (Apis mellifera) venom phospholipase A2. The role of alpha 1,3-fucosylation of the asparagine-bound N-acetylglucosamine.

Authors:  C Prenner; L Mach; J Glössl; L März
Journal:  Biochem J       Date:  1992-06-01       Impact factor: 3.857
  3 in total

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