Literature DB >> 29382972

Development and application of a droplet digital polymerase chain reaction (ddPCR) for detection and investigation of African swine fever virus.

Xulong Wu1, Lu Xiao1, Hua Lin1, Shijie Chen1, Miao Yang1, Wei An1, Yin Wang1, Zexiao Yang1, Xueping Yao1, Zizhong Tang1.   

Abstract

The aim of this study was to develop a droplet digital polymerase chain reaction (ddPCR) method to detect African swine fever virus (ASFV). The methods of ASFV real-time PCR and ddPCR were established and optimal reaction conditions were confirmed. Each method was evaluated for linearity, limit of detection, and specificity. The results indicated that ASFV ddPCR had a high degree of linearity (R2 ≥ 0.998) and specificity. The detection limit was 10 copies/reaction, which was approximately a 10-fold greater sensitivity than real-time PCR. This sensitive method could be used as an efficient molecular biology tool to diagnose ASFV, which is very important for preventing the spread of diseases across borders.

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Year:  2018        PMID： 29382972      PMCID： PMC5764039     

Source DB:  PubMed          Journal:  Can J Vet Res        ISSN： 0830-9000            Impact factor:   1.310


  13 in total

1.  Quantitative real-time PCR for determination of microcystin synthetase e copy numbers for microcystis and anabaena in lakes.

Authors:  Jaana Vaitomaa; Anne Rantala; Katrianna Halinen; Leo Rouhiainen; Petra Tallberg; Lena Mokelke; Kaarina Sivonen
Journal:  Appl Environ Microbiol       Date:  2003-12       Impact factor: 4.792

2.  Droplet digital PCR as a useful tool for the quantitative detection of Enterovirus 71.

Authors:  Yan Long Edmund Lui; Eng Lee Tan
Journal:  J Virol Methods       Date:  2014-07-24       Impact factor: 2.014

3.  African swine fever diagnosis update.

Authors:  J M Sánchez-Vizcaíno; L Mur
Journal:  Dev Biol (Basel)       Date:  2013-05-14

4.  Development of a duplex lateral flow assay for simultaneous detection of antibodies against African and Classical swine fever viruses.

Authors:  Patricia Sastre; Teresa Pérez; Sofia Costa; Xiaoping Yang; Alex Räber; Sandra Blome; Katja V Goller; Carmina Gallardo; Istar Tapia; Julia García; Antonio Sanz; Paloma Rueda
Journal:  J Vet Diagn Invest       Date:  2016-07-07       Impact factor: 1.279

5.  Comparison of next-generation droplet digital PCR (ddPCR) with quantitative PCR (qPCR) for enumeration of Cryptosporidium oocysts in faecal samples.

Authors:  Rongchang Yang; Andrea Paparini; Paul Monis; Una Ryan
Journal:  Int J Parasitol       Date:  2014-09-16       Impact factor: 3.981

6.  Detection of African swine fever, classical swine fever, and foot-and-mouth disease viruses in swine oral fluids by multiplex reverse transcription real-time polymerase chain reaction.

Authors:  Frederic R Grau; Megan E Schroeder; Erin L Mulhern; Michael T McIntosh; Mangkey A Bounpheng
Journal:  J Vet Diagn Invest       Date:  2015-03       Impact factor: 1.279

Review 7.  African swine fever virus infection in Ornithodoros ticks.

Authors:  Thomas G Burrage
Journal:  Virus Res       Date:  2012-10-17       Impact factor: 3.303

8.  Droplet digital polymerase chain reaction for DNMT3A and IDH1/2 mutations to improve early detection of acute myeloid leukemia relapse after allogeneic hematopoietic stem cell transplantation.

Authors:  Chiara Brambati; Silvia Galbiati; Elisabetta Xue; Cristina Toffalori; Lara Crucitti; Raffaella Greco; Elisa Sala; Alessandra Crippa; Lorenza Chiesa; Nadia Soriani; Benedetta Mazzi; Cristina Tresoldi; Maria Teresa Lupo Stanghellini; Jacopo Peccatori; Matteo G Carrabba; Massimo Bernardi; Maurizio Ferrari; Vito Lampasona; Fabio Ciceri; Luca Vago
Journal:  Haematologica       Date:  2015-12-24       Impact factor: 11.047

9.  Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification.

Authors:  Leonardo B Pinheiro; Victoria A Coleman; Christopher M Hindson; Jan Herrmann; Benjamin J Hindson; Somanath Bhat; Kerry R Emslie
Journal:  Anal Chem       Date:  2011-12-21       Impact factor: 6.986

10.  Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.

Authors:  Lars Gerdes; Azuka Iwobi; Ulrich Busch; Sven Pecoraro
Journal:  Biomol Detect Quantif       Date:  2016-01-07
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  7 in total

1.  Application of portable real-time recombinase-aided amplification (rt-RAA) assay in the clinical diagnosis of ASFV and prospective DIVA diagnosis.

Authors:  Zhao-Hua Wang; Pei Li; Xiao Lin; Hong Jia; Yi-Tong Jiang; Xiao-Jia Wang; Shao-Hua Hou
Journal:  Appl Microbiol Biotechnol       Date:  2021-04-09       Impact factor: 4.813

2.  Development and Application of a Duplex Droplet Digital Polymerase Chain Reaction Assay for Detection and Differentiation of EP402R-Deleted and Wild-Type African Swine Fever Virus.

Authors:  Junhai Zhu; Weijun Jian; Yifan Huang; Qi Gao; Fei Gao; Huahan Chen; Guihong Zhang; Ming Liao; Wenbao Qi
Journal:  Front Vet Sci       Date:  2022-06-06

3.  SPR-Based Detection of ASF Virus in Cells.

Authors:  Alessandro Capo; Alessia Calabrese; Maciej Frant; Marek Walczak; Anna Szczotka-Bochniarz; Georgios Manessis; Ioannis Bossis; Maria Staiano; Sabato D'Auria; Antonio Varriale
Journal:  Int J Mol Sci       Date:  2022-07-05       Impact factor: 6.208

4.  A Multiplex Crystal Digital PCR for Detection of African Swine Fever Virus, Classical Swine Fever Virus, and Porcine Reproductive and Respiratory Syndrome Virus.

Authors:  Kaichuang Shi; Yating Chen; Yanwen Yin; Feng Long; Shuping Feng; Huixin Liu; Sujie Qu; Hongbin Si
Journal:  Front Vet Sci       Date:  2022-06-24

5.  Characterization of Recombinant Adeno-Associated Viruses (rAAVs) for Gene Therapy Using Orthogonal Techniques.

Authors:  Liam Cole; Diogo Fernandes; Maryam T Hussain; Michael Kaszuba; John Stenson; Natalia Markova
Journal:  Pharmaceutics       Date:  2021-04-20       Impact factor: 6.321

6.  Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method.

Authors:  John Charles Rotondo; Lucia Oton-Gonzalez; Chiara Mazziotta; Carmen Lanzillotti; Maria Rosa Iaquinta; Mauro Tognon; Fernanda Martini
Journal:  Front Microbiol       Date:  2020-11-19       Impact factor: 5.640

Review 7.  Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management.

Authors:  Dexin Zeng; Bingxu Qian; Yunfei Li; Kai Zong; Wanqing Peng; Kai Liao; Xiaofeng Yu; Juanjuan Sun; Xiaying Lv; Liu Ding; Manman Wang; Tingting Zhou; Yuan Jiang; Jinming Li; Feng Xue; Xiaodong Wu; Jianjun Dai
Journal:  Front Microbiol       Date:  2022-09-30       Impact factor: 6.064
  7 in total

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