| Literature DB >> 29382202 |
Qiong Wu1, Suyog Madhav Amrutkar1, Fangwei Shao1.
Abstract
We developed an enzyme-free, chemical method to selectively label the epigenetic base, 5-hydroxymethylcytosine (hmC) with versatile sulfinate reagents in aqueous solvent under mild reaction conditions. This method allows efficient single step conjugation of biotin to hmC site in DNA for enrichment and pull down assays.Entities:
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Year: 2018 PMID： 29382202 DOI： 10.1021/acs.bioconjchem.7b00826
Source DB: PubMed Journal: Bioconjug Chem ISSN： 1043-1802 Impact factor: 4.774