| Literature DB >> 29381767 |
Hanna Ainelo, Andrio Lahesaare, Annika Teppo, Maia Kivisaar, Riho Teras.
Abstract
[This corrects the article DOI: 10.1371/journal.pone.0185482.].Entities:
Year: 2018 PMID： 29381767 PMCID： PMC5790286 DOI： 10.1371/journal.pone.0192336
Source DB: PubMed Journal: PLoS One ISSN： 1932-6203 Impact factor: 3.240
B-galactosidase activity (Miller units) expressed from different length lapA promoter constructs.
| Construct | 4 h | 18 h | ||
|---|---|---|---|---|
| PSm | PSm∆rpoS | PSm | PSm∆rpoS | |
| pBLKT | 0.36 (0.05) a | 0.58 (0.30) a | 0.98 (0.05) b | 1.09 (0.13) b |
| pB_PlapA1 | 0.8 (0.2) a | |||
| pB_PlapA1-2 | 9.9 (2.5) a | |||
| pB_PlapA1-3 | 570.6 (46.4) b | |||
| pB_PlapA1-4 | 501.5 (95.7) bc | |||
| pB_PlapA1-5 | 567.7 (21.4) bc | |||
| pB_PlapA1-6 | 861.7 (53.0) d | |||
| pB_PlapA1-7 | 457.5 (95.4) c | |||
| pB_PlapA1-8 | 516.7 (37.7) bc | |||
| pB_PlapA2 | 9.7 (1.6) a | 7.7 (1.0) a | 18.4 (3.3) b | 16.9 (2.4) b |
| pB_PlapA2mut | 7.4 (1.2) a | 15.1 (3.0) b | ||
| pB_PlapA3 | 398.4 (31.2) b | 480.2 (44.0) b | 1217.8 (106.5) c | 1443.3 (90.0) d |
| pB_PlapA3mut | 28.7 (2.6) a | 71.0 (4.4) a | ||
| pB_PlapA4 | 70.4 (9.0) b | 67.7 (3.2) b | 374.5 (74.2) c | 385.5 (113.8) c |
| pB_PlapA4mut | 2.4 (0.6) a | 10.8 (2.1) ab | ||
| pB_PlapA5 | 20.9 (2.3) b | 22.6 (4.1) b | 91.2 (5.5) d | 83.9 (9.0) d |
| pB_PlapA5mut | 10.2 (0.8) a | 38.8 (2.9) c | ||
| pB_PlapA6 | 8.1 (1.1) b | 8.7 (1.3) b | 34.4 (1.5) d | 19.7 (1.5) c |
| pB_PlapA6mut | 0.4 (0.1) a | 2.4 (0.3) a | ||
| pB_PlapA7 | 8.8 (1.5) b | 7.6 (0.3) b | 37.6 (4.0) d | 24.0 (1.5) c |
| pB_PlapA7mut | 1.1 (0.1) a | 2.8 (0.3) ab | ||
| pB_PlapA8 | 32.6 (2.2) b | 35.9 (2.4) b | 163.2 (7.5) d | 123.0 (11.5) c |
| pB_PlapA8mut | 12.0 (1.4) a | 33.1 (7.4) b | ||
*The promoterless pBLKT does not contain lapA upstream DNA.
Data from at least 5 independent measurements is shown. 95% confidence intervals are shown in parentheses. Not determined activities are marked with the “-”symbol. Groups of data analysed separately are divided by bold horizontal lines and background colour. Letters a-e depict different homogeneity groups according to ANOVA post hoc Bonferroni test. Identical letters denote non-significant differences (P>0.05) between averages of β-galactosidase activity.