Dmitriy I Dogadov1, Lydia I Korzaya1, Anastasia A Karlsen2,3, Karen K Kyuregyan2,3. 1. Scientific Research Institute of Medical Primatology, Sochi, Russia. 2. Russian Medical Academy of Postgraduate Education, Moscow, Russia. 3. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.
Abstract
BACKGROUND: The aim of this study was to assess prevalence and genetic variability of hepatitis A virus (HAV) isolates in monkeys born and kept at Adler Primate Center, as well as in imported animals. METHODS: The fecal samples from various species of monkeys (n = 119) were studied using reverse transcription seminested PCR, sequencing, and phylogenetic analysis. RESULTS: HAV RNA was detected in 2 Macaca mulatta and 1 Macaca fascicularis (3.8%) kept at Adler Primate Center (n = 79) and in 11 (27.5%) Chlorocebus pygerythrus (n = 40) imported from Tanzania. Phylogenetic analysis demonstrated that all HAV strains belonged to simian genotype V, but differed from the prototype genotype V strain (AGM-27) by 5.4%-5.5%. Sequences isolated in this study differed by only 0.1%, suggesting a common source of infection. CONCLUSIONS: This study demonstrated the asymptomatic circulation of HAV genotype V among the monkeys at Adler Primate Center, and it indicated the significant genetic diversity within this HAV genotype.
BACKGROUND: The aim of this study was to assess prevalence and genetic variability of hepatitis A virus (HAV) isolates in monkeys born and kept at Adler Primate Center, as well as in imported animals. METHODS: The fecal samples from various species of monkeys (n = 119) were studied using reverse transcription seminested PCR, sequencing, and phylogenetic analysis. RESULTS:HAV RNA was detected in 2 Macaca mulatta and 1 Macaca fascicularis (3.8%) kept at Adler Primate Center (n = 79) and in 11 (27.5%) Chlorocebus pygerythrus (n = 40) imported from Tanzania. Phylogenetic analysis demonstrated that all HAV strains belonged to simian genotype V, but differed from the prototype genotype V strain (AGM-27) by 5.4%-5.5%. Sequences isolated in this study differed by only 0.1%, suggesting a common source of infection. CONCLUSIONS: This study demonstrated the asymptomatic circulation of HAV genotype V among the monkeys at Adler Primate Center, and it indicated the significant genetic diversity within this HAV genotype.
Authors: Ivan Balansard; Lorna Cleverley; Keith L Cutler; Mats G Spångberg; Kevin Thibault-Duprey; Jan Am Langermans Journal: Lab Anim Date: 2019-05-08 Impact factor: 2.471