Adriana Fernandes Da Silva1, Marcelo Rocha Marques2, Wellington Luiz De Oliveira Da Rosa3, Sandra Beatriz Chaves Tarquinio4, Pedro Luiz Rosalen5, Silvana Pereira Barros6. 1. Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas (UFPel), Pelotas, Brazil. adriana@ufpel.edu.br. 2. Department of Morphology, School of Dentistry, State University of Campinas (UNICAMP), Piracicaba, Brazil. 3. Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas (UFPel), Pelotas, Brazil. 4. Department of Semiology and Clinics, School of Dentistry, Federal University of Pelotas (UFPel), Pelotas, Brazil. 5. Department of Physiological Sciences, School of Dentistry, State University of Campinas (UNICAMP), Piracicaba, Brazil. 6. Center for Oral and Systemic Diseases, Department of Periodontology, School of Dentistry, University of North Carolina (UNC), Chapel Hill, USA.
Abstract
OBJECTIVES: The purposes of this study were to evaluate a model of slow caries progression and to investigate the performance of a self-etch adhesive system for partial caries removal. MATERIALS AND METHODS: Rat molars were infected with Streptococcus sobrinus 6715 culture. Different time points were analyzed: days 78, 85, and 95 (± 2). After this, the samples were processed for morphological analysis. Additionally, the first molars were restored with zinc oxide and eugenol (IRM™; Dentsply; Brazil) or adhesive system (Clearfil SE Bond™; Kuraray Medical; Japan) 78 days after caries induction. After, 3 or 15 days post-treatment, the animals were euthanized, and their mandibles were processed for morphological analysis, classified by means of scores, and submitted to statistical analysis. Subsequently, immunohistochemical analysis was performed for osteonectin (OSN) and transforming growth factor-ß1 (TGF-ß1) expression. RESULTS: According to the caries induction model used, on day 95 greater inflammatory infiltration (p < 0.001), and more extensive degradation of secondary/primary dentin were demonstrated than on day 78 (p < 0.05). Furthermore, the restorative materials presented similar performance (p > 0.05) and proved to be fundamental to control the carious lesion. The TGF-ß1 and OSN were shown to be active during the caries process. CONCLUSIONS: The slow caries lesion model was feasible for morphological analysis of the dentin-pulp complex. The self-etch adhesive system triggered no acute inflammatory infiltration or pulp necrosis, instead it seemed to stimulate early pulp repair. CLINICAL RELEVANCE: Clearfil SE Bond™ applied directly on caries-affected dentin did not predispose to pulp inflammation; instead, it appeared to provide early biological benefits.
OBJECTIVES: The purposes of this study were to evaluate a model of slow caries progression and to investigate the performance of a self-etch adhesive system for partial caries removal. MATERIALS AND METHODS:Rat molars were infected with Streptococcus sobrinus 6715 culture. Different time points were analyzed: days 78, 85, and 95 (± 2). After this, the samples were processed for morphological analysis. Additionally, the first molars were restored with zinc oxide and eugenol (IRM™; Dentsply; Brazil) or adhesive system (Clearfil SE Bond™; Kuraray Medical; Japan) 78 days after caries induction. After, 3 or 15 days post-treatment, the animals were euthanized, and their mandibles were processed for morphological analysis, classified by means of scores, and submitted to statistical analysis. Subsequently, immunohistochemical analysis was performed for osteonectin (OSN) and transforming growth factor-ß1 (TGF-ß1) expression. RESULTS: According to the caries induction model used, on day 95 greater inflammatory infiltration (p < 0.001), and more extensive degradation of secondary/primary dentin were demonstrated than on day 78 (p < 0.05). Furthermore, the restorative materials presented similar performance (p > 0.05) and proved to be fundamental to control the carious lesion. The TGF-ß1 and OSN were shown to be active during the caries process. CONCLUSIONS: The slow caries lesion model was feasible for morphological analysis of the dentin-pulp complex. The self-etch adhesive system triggered no acute inflammatory infiltration or pulp necrosis, instead it seemed to stimulate early pulp repair. CLINICAL RELEVANCE: Clearfil SE Bond™ applied directly on caries-affected dentin did not predispose to pulp inflammation; instead, it appeared to provide early biological benefits.
Authors: M Yoshiyama; F R Tay; J Doi; Y Nishitani; T Yamada; K Itou; R M Carvalho; M Nakajima; D H Pashley Journal: J Dent Res Date: 2002-08 Impact factor: 6.116
Authors: Wellington Luiz de Oliveira da Rosa; Tiago Machado da Silva; Flávio Fernando Demarco; Evandro Piva; Adriana Fernandes da Silva Journal: J Biomed Mater Res A Date: 2017-01-18 Impact factor: 4.396