Wahyu Widyaningsih1, Suwidjiyo Pramono2, Sitarina Widyarini3. 1. Department of Pharmacy, Faculty of Pharmacy, Universitas Ahmad Dahlan, 55281, Yogyakarta, Indonesia. 2. Laboratory of Phytochemistry, Faculty of Pharmacy, Universitas Gadjah Mada, 55281, Yogyakarta, Indonesia. 3. Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, 55281, Yogyakarta, Indonesia.
Abstract
BACKGROUND: Reactive oxygen species (ROS) play a major role in myocardial damage during acute myocardial infarction (AMI). This study aimed to determine the antioxidant and antiapoptotic activities of an ethanolic extract from Ulva lactuca L. (EEUL) against AMI. METHODS: Thirty-six male Wistar rats were divided into six groups: one control group and five treatment groups. Treatment group II was given 85 mg/kg body weight (BW) of isoproterenol (ISO). Group III, IV and V were given ISO and EEUL at 250, 500 and 750 mg/kg BW, respectively. Group VI were given 10 mg/kg BW of ISO and melatonin. EEUL and melatonin were orally administered for 28 days. ISO was injected subcutaneously on day 29 and 30 to chemically induce AMI. On day 31, blood was collected for antioxidant assay and heart tissues were collected for histological examination. RESULTS: The activity of catalase (CAT), an endogenous antioxidant, in the EEUL-treatment groups was significantly increased compared to the ISO-treatment group (P < 0.001). The EEUL-treatment groups showed significantly decreased expression of caspase-3 (P < 0.001) and better myocardial tissue morphology. CONCLUSION: EEUL possibly protects against AMI because of its antioxidant and antiapoptotic properties.
BACKGROUND: Reactive oxygen species (ROS) play a major role in myocardial damage during acute myocardial infarction (AMI). This study aimed to determine the antioxidant and antiapoptotic activities of an ethanolic extract from Ulva lactuca L. (EEUL) against AMI. METHODS: Thirty-six male Wistar rats were divided into six groups: one control group and five treatment groups. Treatment group II was given 85 mg/kg body weight (BW) of isoproterenol (ISO). Group III, IV and V were given ISO and EEUL at 250, 500 and 750 mg/kg BW, respectively. Group VI were given 10 mg/kg BW of ISO and melatonin. EEUL and melatonin were orally administered for 28 days. ISO was injected subcutaneously on day 29 and 30 to chemically induce AMI. On day 31, blood was collected for antioxidant assay and heart tissues were collected for histological examination. RESULTS: The activity of catalase (CAT), an endogenous antioxidant, in the EEUL-treatment groups was significantly increased compared to the ISO-treatment group (P < 0.001). The EEUL-treatment groups showed significantly decreased expression of caspase-3 (P < 0.001) and better myocardial tissue morphology. CONCLUSION: EEUL possibly protects against AMI because of its antioxidant and antiapoptotic properties.
Authors: D Hevia; C Botas; R M Sainz; I Quiros; D Blanco; D X Tan; C Gomez-Cordoves; J C Mayo Journal: J Chromatogr A Date: 2010-01-04 Impact factor: 4.759