Lauren Housley1,2, Armando Alcazar Magana3,4, Anna Hsu1, Laura M Beaver1, Carmen P Wong1, Jan F Stevens3,5, Jaewoo Choi3, Yuan Jiang6, Deborah Bella1, David E Williams3,7, Claudia S Maier4, Jackilen Shannon8, Roderick H Dashwood9, Emily Ho1,3,9,10. 1. School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR, USA. 2. Department of Nutrition and Food Science, California State University, Chico, CA, USA. 3. Linus Pauling Institute, Oregon State University, Corvallis, OR, USA. 4. Department of Chemistry, Oregon State University, Corvallis, OR, USA. 5. Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, USA. 6. Department of Statistics, Oregon State University, Corvallis, OR, USA. 7. Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, USA. 8. Department of Public Health & Preventive Medicine, Oregon Health and Science University, Portland, OR, USA. 9. Center for Epigenetics & Disease Prevention, Texas A&M Health Science Center, Houston, TX, USA. 10. Moore Family Center for Whole Grain Foods, Nutrition and Preventive Health, Oregon State University, Corvallis, OR, USA.
Abstract
SCOPE: Several lines of evidence suggest that the consumption of cruciferous vegetables is beneficial to human health. Yet, underlying mechanisms and key molecular targets that are involved with achieving these benefits in humans are still not fully understood. To accelerate this research, we conduct a human study to identify potential molecular targets of crucifers for further study. This study aims to characterize plasma metabolite profiles in humans before and after consuming fresh broccoli sprouts (a rich dietary source of bioactive sulforaphane). METHODS AND RESULTS: Ten healthy adults consume fresh broccoli sprouts (containing 200 μmol sulforaphane equivalents) at time 0 and provide blood samples at 0, 3, 6, 12, 24, and 48 h. An untargeted metabolomics screen reveals that levels of several plasma metabolites are significantly different before and after sprout intake, including fatty acids (14:0, 14:1, 16:0, 16:1, 18:0, and 18:1), glutathione, glutamine, cysteine, dehydroepiandrosterone, and deoxyuridine monophosphate. Evaluation of all time points is conducted using paired t-test (R software) and repeated measures analysis of variance for a within-subject design (Progenesis QI). CONCLUSION: This investigation identifies several potential molecular targets of crucifers that may aid in studying established and emerging health benefits of consuming cruciferous vegetables and related bioactive compounds.
SCOPE: Several lines of evidence suggest that the consumption of cruciferous vegetables is beneficial to human health. Yet, underlying mechanisms and key molecular targets that are involved with achieving these benefits in humans are still not fully understood. To accelerate this research, we conduct a human study to identify potential molecular targets of crucifers for further study. This study aims to characterize plasma metabolite profiles in humans before and after consuming fresh broccoli sprouts (a rich dietary source of bioactive sulforaphane). METHODS AND RESULTS: Ten healthy adults consume fresh broccoli sprouts (containing 200 μmol sulforaphane equivalents) at time 0 and provide blood samples at 0, 3, 6, 12, 24, and 48 h. An untargeted metabolomics screen reveals that levels of several plasma metabolites are significantly different before and after sprout intake, including fatty acids (14:0, 14:1, 16:0, 16:1, 18:0, and 18:1), glutathione, glutamine, cysteine, dehydroepiandrosterone, and deoxyuridine monophosphate. Evaluation of all time points is conducted using paired t-test (R software) and repeated measures analysis of variance for a within-subject design (Progenesis QI). CONCLUSION: This investigation identifies several potential molecular targets of crucifers that may aid in studying established and emerging health benefits of consuming cruciferous vegetables and related bioactive compounds.
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