Luis Exequiel Ibarra1, Gabriela Valeria Porcal2,3, Lorena Paola Macor2,3, Rodrigo Andrés Ponzio3,4, Ramiro Martin Spada2,3, Carolina Lorente5, Carlos Alberto Chesta2,3, Viviana Alicia Rivarola1, Rodrigo Emiliano Palacios2,3. 1. Universidad Nacional de Río Cuarto y CONICET, Instituto de Biotecnología Ambiental y Salud (INBIAS), Dto. Biología Molecular, Facultad de Ciencias Exactas Fisicoquímicas y Naturales, Río Cuarto (5800), Córdoba, Argentina. 2. Universidad Nacional de Río Cuarto y CONICET, Dto. Química, Facultad de Ciencias Exactas Fisicoquímicas y Naturales, Río Cuarto (5800), Córdoba, Argentina. 3. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados (IITEMA), UNRC-CONICET, Argentina. 4. Universidad Nacional de Río Cuarto y CONICET, Dto. Física, Facultad de Ciencias Exactas Fisicoquímicas y Naturales, Río Cuarto (5800), Córdoba, Argentina. 5. Universidad Nacional de La Plata y CONICET, Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Dto. Química, Facultad de Ciencias Exactas, CCT La Plata CONICET, La Plata (1900), Buenos Aires, Argentina.
Abstract
AIM: Assess biocompatibility, uptake and photodynamic therapy (PDT) mechanism of metallated porphyrin doped conjugated polymer nanoparticles (CPNs) in human brain and colorectal tumor cells and macrophages. MATERIALS & METHODS: CPNs were developed employing 9,9-dioctylfluorene-alt-benzothiadiazole, an amphiphilic polymer (PS-PEG-COOH), and platinum octaethylporphyrin. T98G, SW480 and RAW 264.7 cell lines were exposed to CPNs to assess uptake and intracellular localization. Additionally, a PDT protocol using CPNs was employed for the in vitro killing of cancer and macrophage cell lines. RESULTS & CONCLUSION: CPNs were well incorporated into glioblastoma and macrophage cells with localization in lysosomes. SW480 cells were less efficient incorporating CPNs with localization in the plasma membrane. In all cell lines PDT treatment was efficient inducing oxidative stress that triggered apoptosis.
AIM: Assess biocompatibility, uptake and photodynamic therapy (PDT) mechanism of metallated porphyrin doped conjugated polymer nanoparticles (CPNs) in human brain and colorectal tumor cells and macrophages. MATERIALS & METHODS:CPNs were developed employing 9,9-dioctylfluorene-alt-benzothiadiazole, an amphiphilic polymer (PS-PEG-COOH), and platinum octaethylporphyrin. T98G, SW480 and RAW 264.7 cell lines were exposed to CPNs to assess uptake and intracellular localization. Additionally, a PDT protocol using CPNs was employed for the in vitro killing of cancer and macrophage cell lines. RESULTS & CONCLUSION:CPNs were well incorporated into glioblastoma and macrophage cells with localization in lysosomes. SW480 cells were less efficient incorporating CPNs with localization in the plasma membrane. In all cell lines PDT treatment was efficient inducing oxidative stress that triggered apoptosis.