| Literature DB >> 2937443 |
H H van Vliet, M C Kappers-Klunne, J Abels.
Abstract
Increased binding of IgM to donor platelets was detected in serum of a patient demonstrating the phenomenon of pseudothrombocytopenia. The elevated IgM binding was dependent on EDTA concentration and temperature, and could not be demonstrated when platelets of patients with Glanzmann's disease were used. Immunoblotting and crossed immunoelectrophoresis showed that the IgM antibody reacted with platelet glycoprotein GP IIb. Evidence is presented that the antigenic binding site on GP IIb is made accessible to the antibody by means of the effect of EDTA on the Ca2+ dependent GP IIb/IIIa complex.Entities:
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Year: 1986 PMID： 2937443 DOI： 10.1111/j.1365-2141.1986.tb02962.x
Source DB: PubMed Journal: Br J Haematol ISSN： 0007-1048 Impact factor: 6.998