Yan-Ni Chen1, Hui-Ying Du2, Zhuo-Yue Shi3, Li He2, Yu-Ying He4, Duan Wang5. 1. Department of Health Care, Xi'an Children's Hospital, Xi'an Jiaotong University, 69 Xijuyuanxiang, Xi'an, 710002, China. chenyannichil@163.com. 2. Department of Health Care, Xi'an Children's Hospital, Xi'an Jiaotong University, 69 Xijuyuanxiang, Xi'an, 710002, China. 3. Department of Biology, College of Liberal Arts and Science, The University of Iowa, Iowa, USA. 4. Xi'an Maternal and Child Health Hospital, 73 West Street, Xi'an, 710002, China. 5. Department of Pediatrics, Shaanxi University of Chinese Medicine, Xianyang, Shannxi, China.
Abstract
BACKGROUND: The pathogenesis of autism spectrum disorders remains elusive and currently there are no diagnostic or predictive biomarkers in autism available. Proteomic profiling has been used in a wide range of neurodevelopmental disorder studies, which could produce deeper perceptions of the molecular bases behind certain disease and potentially becomes useful in discovering biomarkers in autism spectrum disorders. METHODS: Serum samples were collected from autistic children about 3 years old in age (n = 32) and healthy controls (n = 20) in similar age and gender. The samples were identified specific proteins that are differentially expressed by magnetic bead-based pre-fractionation and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF-MS). RESULTS: Eight protein peaks were significantly different in autistic children from the healthy controls (P < 0.0001). The two peaks with the most significant differences were 6428 and 7758 Da in size. CONCLUSION: According to differences in serum protein profiles between the autistic children and healthy controls, this study identified a set of differentially expressed proteins those are significant for further evaluation and might function as biomarkers in autism.
BACKGROUND: The pathogenesis of autism spectrum disorders remains elusive and currently there are no diagnostic or predictive biomarkers in autism available. Proteomic profiling has been used in a wide range of neurodevelopmental disorder studies, which could produce deeper perceptions of the molecular bases behind certain disease and potentially becomes useful in discovering biomarkers in autism spectrum disorders. METHODS: Serum samples were collected from autisticchildren about 3 years old in age (n = 32) and healthy controls (n = 20) in similar age and gender. The samples were identified specific proteins that are differentially expressed by magnetic bead-based pre-fractionation and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF-MS). RESULTS: Eight protein peaks were significantly different in autisticchildren from the healthy controls (P < 0.0001). The two peaks with the most significant differences were 6428 and 7758 Da in size. CONCLUSION: According to differences in serum protein profiles between the autisticchildren and healthy controls, this study identified a set of differentially expressed proteins those are significant for further evaluation and might function as biomarkers in autism.
Entities:
Keywords:
Autism; Matrix-assisted laser desorption ionization time-of-flight mass spectrometry; Proteomic profiling; Weak cation-exchange chromatography magnetic beads
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