Ronghua Wang1,2, Yi Mei3, Liang Xu1, Xianwen Zhu4, Yan Wang1, Jun Guo3, Liwang Liu5. 1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. 2. Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Jinan, 250100, People's Republic of China. 3. Yancheng Academy of Agricultural Sciences, Yancheng, 224002, Jiangsu, People's Republic of China. 4. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA. 5. National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. nauliulw@njau.edu.cn.
Abstract
MAIN CONCLUSION: Differential abundance protein species (DAPS) involved in reducing damage and enhancing thermotolerance in radish were firstly identified. Proteomic analysis and omics association analysis revealed a HS-responsive regulatory network in radish. Heat stress (HS) is a major destructive factor influencing radish production and supply in summer, for radish is a cool season vegetable crop being susceptible to high temperature. In this study, the proteome changes of radish taproots under 40 °C treatment at 0 h (Control), 12 h (Heat12) and 24 h (Heat24) were analyzed using iTRAQ (Isobaric Tag for Relative and Absolute Quantification) approach. In total, 2258 DAPS representing 1542 differentially accumulated uniprotein species which respond to HS were identified. A total of 604, 910 and 744 DAPS was detected in comparison of Control vs. Heat12, Control vs. Heat24, and Heat12 vs. Heat24, respectively. Gene ontology and pathway analysis showed that annexin, ubiquitin-conjugating enzyme, ATP synthase, heat shock protein (HSP) and other stress-related proteins were predominately enriched in signal transduction, stress and defense pathways, photosynthesis and energy metabolic pathways, working cooperatively to reduce stress-induced damage in radish. Based on iTRAQ combined with the transcriptomics analysis, a schematic model of a sequential HS-responsive regulatory network was proposed. The initial sensing of HS occurred at the plasma membrane, and then key components of stress signal transduction triggered heat-responsive genes in the plant protective metabolism to re-establish homeostasis and enhance thermotolerance. These results provide new insights into characteristics of HS-responsive DAPS and facilitate dissecting the molecular mechanisms underlying heat tolerance in radish and other root crops.
MAIN CONCLUSION: Differential abundance protein species (DAPS) involved in reducing damage and enhancing thermotolerance in radish were firstly identified. Proteomic analysis and omics association analysis revealed a HS-responsive regulatory network in radish. Heat stress (HS) is a major destructive factor influencing radish production and supply in summer, for radish is a cool season vegetable crop being susceptible to high temperature. In this study, the proteome changes of radish taproots under 40 °C treatment at 0 h (Control), 12 h (Heat12) and 24 h (Heat24) were analyzed using iTRAQ (Isobaric Tag for Relative and Absolute Quantification) approach. In total, 2258 DAPS representing 1542 differentially accumulated uniprotein species which respond to HS were identified. A total of 604, 910 and 744 DAPS was detected in comparison of Control vs. Heat12, Control vs. Heat24, and Heat12 vs. Heat24, respectively. Gene ontology and pathway analysis showed that annexin, ubiquitin-conjugating enzyme, ATP synthase, heat shock protein (HSP) and other stress-related proteins were predominately enriched in signal transduction, stress and defense pathways, photosynthesis and energy metabolic pathways, working cooperatively to reduce stress-induced damage in radish. Based on iTRAQ combined with the transcriptomics analysis, a schematic model of a sequential HS-responsive regulatory network was proposed. The initial sensing of HS occurred at the plasma membrane, and then key components of stress signal transduction triggered heat-responsive genes in the plant protective metabolism to re-establish homeostasis and enhance thermotolerance. These results provide new insights into characteristics of HS-responsive DAPS and facilitate dissecting the molecular mechanisms underlying heat tolerance in radish and other root crops.
Entities:
Keywords:
Differential abundance protein species (DAPS); Heat stress; Thermotolerance; iTRAQ
Authors: David Bungard; Benjamin J Fuerth; Ping-Yao Zeng; Brandon Faubert; Nancy L Maas; Benoit Viollet; David Carling; Craig B Thompson; Russell G Jones; Shelley L Berger Journal: Science Date: 2010-07-15 Impact factor: 47.728
Authors: Oswaldo Valdés-López; Josef Batek; Nicolas Gomez-Hernandez; Cuong T Nguyen; Mariel C Isidra-Arellano; Ning Zhang; Trupti Joshi; Dong Xu; Kim K Hixson; Karl K Weitz; Joshua T Aldrich; Ljiljana Paša-Tolić; Gary Stacey Journal: Front Plant Sci Date: 2016-04-25 Impact factor: 5.753
Authors: Ander Castander-Olarieta; Cátia Pereira; Itziar A Montalbán; Vera M Mendes; Sandra Correia; Sonia Suárez-Álvarez; Bruno Manadas; Jorge Canhoto; Paloma Moncaleán Journal: Front Plant Sci Date: 2021-04-12 Impact factor: 5.753