Literature DB >> 29367940

Ex vivo Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery.

Damayanti Chakraborty1, Masanaga Muto1, Michael J Soares1,2.   

Abstract

In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of 'gain-of-function' and 'loss-of-function' blastocyst manipulations can be evaluated with in vitro outgrowth assays or following transfer to pseudopregnant rats.

Entities:  

Keywords:  Blastocyst; Lentiviral vector; Placenta; Trophoblast

Year:  2017        PMID： 29367940      PMCID： PMC5777579          DOI： 10.21769/BioProtoc.2652

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  18 in total

Review 1.  Placenta: the forgotten organ.

Authors:  Emin Maltepe; Susan J Fisher
Journal:  Annu Rev Cell Dev Biol       Date:  2015-10-05       Impact factor: 13.827

Review 2.  Rat placentation: an experimental model for investigating the hemochorial maternal-fetal interface.

Authors:  M J Soares; D Chakraborty; M A Karim Rumi; T Konno; S J Renaud
Journal:  Placenta       Date:  2012-01-28       Impact factor: 3.481

3.  Phenotypic analysis of the rat placenta.

Authors:  Rupasri Ain; Toshihiro Konno; Lindsey N Canham; Michael J Soares
Journal:  Methods Mol Med       Date:  2006

Review 4.  Development of structures and transport functions in the mouse placenta.

Authors:  Erica D Watson; James C Cross
Journal:  Physiology (Bethesda)       Date:  2005-06

5.  Trophoblast-specific gene manipulation using lentivirus-based vectors.

Authors:  Pantelis Georgiades; Brian Cox; Marina Gertsenstein; Kallayanee Chawengsaksophak; Janet Rossant
Journal:  Biotechniques       Date:  2007-03       Impact factor: 1.993

6.  FOSL1 is integral to establishing the maternal-fetal interface.

Authors:  Lindsey N Kent; M A Karim Rumi; Kaiyu Kubota; Dong-Soo Lee; Michael J Soares
Journal:  Mol Cell Biol       Date:  2011-09-26       Impact factor: 4.272

Review 7.  Comparative developmental anatomy of the murine and human definitive placentae.

Authors:  P Georgiades; A C Ferguson-Smith; G J Burton
Journal:  Placenta       Date:  2002-01       Impact factor: 3.481

8.  HIF-KDM3A-MMP12 regulatory circuit ensures trophoblast plasticity and placental adaptations to hypoxia.

Authors:  Damayanti Chakraborty; Wei Cui; Gracy X Rosario; Regan L Scott; Pramod Dhakal; Stephen J Renaud; Makoto Tachibana; M A Karim Rumi; Clifford W Mason; Adam J Krieg; Michael J Soares
Journal:  Proc Natl Acad Sci U S A       Date:  2016-11-02       Impact factor: 11.205

9.  Gestation stage-dependent intrauterine trophoblast cell invasion in the rat and mouse: novel endocrine phenotype and regulation.

Authors:  Rupasri Ain; Lindsey N Canham; Michael J Soares
Journal:  Dev Biol       Date:  2003-08-01       Impact factor: 3.582

10.  Lentiviral Vector-Mediated Complementation Restored Fetal Viability but Not Placental Hyperplasia in Plac1-Deficient Mice.

Authors:  Masanaga Muto; Yoshitaka Fujihara; Tomohiro Tobita; Daiji Kiyozumi; Masahito Ikawa
Journal:  Biol Reprod       Date:  2015-11-19       Impact factor: 4.285
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  5 in total

1.  Conservation at the uterine-placental interface.

Authors:  Regan L Scott; Ha T H Vu; Ashish Jain; Khursheed Iqbal; Geetu Tuteja; Michael J Soares
Journal:  Proc Natl Acad Sci U S A       Date:  2022-10-03       Impact factor: 12.779

2.  SUV39H2 controls trophoblast stem cell fate.

Authors:  Lei Wang; Damayanti Chakraborty; Khursheed Iqbal; Michael J Soares
Journal:  Biochim Biophys Acta Gen Subj       Date:  2021-02-05       Impact factor: 4.117

3.  Intersection of regulatory pathways controlling hemostasis and hemochorial placentation.

Authors:  Masanaga Muto; Damayanti Chakraborty; Kaela M Varberg; Ayelen Moreno-Irusta; Khursheed Iqbal; Regan L Scott; Ross P McNally; Ruhul H Choudhury; John D Aplin; Hiroaki Okae; Takahiro Arima; Shoma Matsumoto; Masatsugu Ema; Alan E Mast; Elin Grundberg; Michael J Soares
Journal:  Proc Natl Acad Sci U S A       Date:  2021-12-14       Impact factor: 12.779

Review 4.  Modeling Trophoblast Cell-Guided Uterine Spiral Artery Transformation in the Rat.

Authors:  Vinay Shukla; Michael J Soares
Journal:  Int J Mol Sci       Date:  2022-03-09       Impact factor: 5.923

5.  SATB1 promotion of trophoblast stem cell renewal through regulation of threonine dehydrogenase.

Authors:  Kaiyu Kubota; Khursheed Iqbal; Michael J Soares
Journal:  Biochim Biophys Acta Gen Subj       Date:  2020-10-01       Impact factor: 3.770
  5 in total

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