Recent insights in microbial exopolysaccharide biosynthesis and engineering strategies.

The distinct biosynthesis pathways for microbial exopolysaccharide production provide different engineering strategies to tailor the chemical structures of the final polymers. This review focuses on the latest insights in the various pathways and identifies bottlenecks as well as promising targets for tailoring microbial polysaccharide production. The main engineering strategies includes the combinatorial assembly of glycosyltransferases and engineering of the Wzx and Wzy proteins for flipping of repeating units as well as polymerization. In the case of synthase based polysaccharides, the use of epimerases or engineering approaches of the synthase itself as well as overexpression of c-di-GMP levels is identified as one of the most promising strategies. For sucrase-based biosynthesis, the in vitro production by engineered sucrase enzymes or adjusted production conditions is shown as a very promising method.