Luc Van Puyvelde1, Maoxuan Liu2, Cedrick Veryser3, Wim M De Borggraeve3, Joseph Mungarulire4, Marie Jeanne Mukazayire4, Walter Luyten1. 1. Department of Biology, Animal Physiology and Neurobiology Section, KU Leuven, Naamsestraat 59, box 2465, 3000 Leuven, Belgium. 2. Department of Biology, Animal Physiology and Neurobiology Section, KU Leuven, Naamsestraat 59, box 2465, 3000 Leuven, Belgium; Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Herestraat 49, box 921, 3000 Leuven, Belgium. Electronic address: maoxuan.liu@kuleuven.be. 3. Department of Chemistry, Molecular Design and Synthesis, KU Leuven, Celestijnenlaan 200F, box 2404, 3000 Leuven, Belgium. 4. National Industrial Research and Development Agency, Kigali City Tower, 13th Floor, PO box 273, Kigali, Rwanda.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Tetradenia (T.) riparia (Hochst.) Codd (Lamiaceae), formerly known as Iboza riparia (Hochst.) N.E.Br., is one of the most frequently used medicinal plants in traditional Rwandese medicine. It was used as a remedy against a wide range of diseases including malaria, angina, yaws, dental abscesses, headache, worm infections and several kinds of fevers and aches. AIM OF THE STUDY: This study aims to identify the compounds active against helminths from Tetradenia riparia. METHODS: A bioassay-guided isolation of anthelmintic compounds from the leaves of Tetradenia riparia was performed using a Caenorhabditis elegans (C. elegans) testing model. RESULTS: The bioassay-guided isolation led to one active compound, i.e. 8(14),15-sandaracopimaradiene-7α,18-diol. Its IC50 value was 5.4 ± 0.9 µg/mL (17.8 ± 2.9 µM). CONCLUSIONS: We identified the bioactive compound from Tetradenia riparia responsible for its anthelmintic activity: 8(14),15-sandaracopimaradiene-7α,18-diol. Although the compound and several of its bioactivities have been described before, this is the first report of its anthelmintic effect.
ETHNOPHARMACOLOGICAL RELEVANCE: Tetradenia (T.) riparia (Hochst.) Codd (Lamiaceae), formerly known as Iboza riparia (Hochst.) N.E.Br., is one of the most frequently used medicinal plants in traditional Rwandese medicine. It was used as a remedy against a wide range of diseases including malaria, angina, yaws, dental abscesses, headache, worm infections and several kinds of fevers and aches. AIM OF THE STUDY: This study aims to identify the compounds active against helminths from Tetradenia riparia. METHODS: A bioassay-guided isolation of anthelmintic compounds from the leaves of Tetradenia riparia was performed using a Caenorhabditis elegans (C. elegans) testing model. RESULTS: The bioassay-guided isolation led to one active compound, i.e. 8(14),15-sandaracopimaradiene-7α,18-diol. Its IC50 value was 5.4 ± 0.9 µg/mL (17.8 ± 2.9 µM). CONCLUSIONS: We identified the bioactive compound from Tetradenia riparia responsible for its anthelmintic activity: 8(14),15-sandaracopimaradiene-7α,18-diol. Although the compound and several of its bioactivities have been described before, this is the first report of its anthelmintic effect.
Authors: Sujogya Kumar Panda; Zilda Cristiani Gazim; Shasank S Swain; Marisa Cassia Vieira de Araujo Bento; Jéssica da Silva Sena; Marie Jeanne Mukazayire; Luc Van Puyvelde; Walter Luyten Journal: Front Pharmacol Date: 2022-06-02 Impact factor: 5.988
Authors: Daniel M Shadrack; Geradius Deogratias; Lucy W Kiruri; Isaac Onoka; John-Mary Vianney; Hulda Swai; Stephen S Nyandoro Journal: J Mol Model Date: 2021-07-08 Impact factor: 1.810
Authors: Aya C Taki; Joseph J Byrne; Tao Wang; Brad E Sleebs; Nghi Nguyen; Ross S Hall; Pasi K Korhonen; Bill C H Chang; Paul Jackson; Abdul Jabbar; Robin B Gasser Journal: Pharmaceuticals (Basel) Date: 2021-06-26