Yunxia Liu1, Jiwu Song2, Jun Zhang3, Liu Yang4, Zongxia Liu5, Xuxia Wang6. 1. Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong, China; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong, China; Institute of Stomatology, Weifang Medical University, Weifang, Shandong, China; Department of Stomatology, The Affiliated Hospital of Weifang Medical University, Weifang, Shandong, China. 2. Department of Stomatology, Weifang People's Hospital, Weifang, Shandong, China. 3. Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong, China; Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong, China. 4. Yantai Stomatological Hospital, Yantai, Shandong, China. 5. Institute of Stomatology, Weifang Medical University, Weifang, Shandong, China. 6. Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong, China; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong, China. Electronic address: Xuxiawang@126.com.
Abstract
OBJECTIVE: This study aimed to investigate the relationship between BTB/POZ domain-containing protein 7 (BTBD7) and fibronectin (FN) expression in salivary adenoid cystic carcinoma (SACC) and the function of BTBD7 in proliferation, migration, and invasion of SACC cells. STUDY DESIGN: The BTBD7 and FN expression in SACC and nontumor salivary tissues as well as SACC cells were characterized by immunohistochemistry and immunofluorescence. The effect of BTBD7 silencing on the proliferation, migration, and invasion of SACC-LM cells were determined by wound healing and transwell invasion assays. RESULTS: The percentages of SACC with positive BTBD7 expression were significantly higher than those of SACC with FN expression. BTBD7 silencing significantly increased the relative levels of FN expression and inhibited the proliferation, migration, and invasion of SACC-LM cells. CONCLUSIONS: The study data indicated that BTBD7 was inversely associated with FN expression in SACC. BTBD7 may inhibit FN expression, but it promotes the proliferation, migration, and invasion of SACC-LM cells. Hence, BTBD7 may be associated with metastasis and a new therapeutic target for intervention of SACC.
OBJECTIVE: This study aimed to investigate the relationship between BTB/POZ domain-containing protein 7 (BTBD7) and fibronectin (FN) expression in salivary adenoid cystic carcinoma (SACC) and the function of BTBD7 in proliferation, migration, and invasion of SACC cells. STUDY DESIGN: The BTBD7 and FN expression in SACC and nontumor salivary tissues as well as SACC cells were characterized by immunohistochemistry and immunofluorescence. The effect of BTBD7 silencing on the proliferation, migration, and invasion of SACC-LM cells were determined by wound healing and transwell invasion assays. RESULTS: The percentages of SACC with positive BTBD7 expression were significantly higher than those of SACC with FN expression. BTBD7 silencing significantly increased the relative levels of FN expression and inhibited the proliferation, migration, and invasion of SACC-LM cells. CONCLUSIONS: The study data indicated that BTBD7 was inversely associated with FN expression in SACC. BTBD7 may inhibit FN expression, but it promotes the proliferation, migration, and invasion of SACC-LM cells. Hence, BTBD7 may be associated with metastasis and a new therapeutic target for intervention of SACC.
Authors: Iulia Cristiana Belulescu; Claudiu Mărgăritescu; Cristiana Iulia Dumitrescu; Maria Cristina Munteanu; Otilia Clara Mărgăritescu Journal: Rom J Morphol Embryol Date: 2020 Impact factor: 1.033