Jean Riou1, Jessica Szuberski2, Christian Godart1, Henri Wajcman3, Jennifer L Oliveira2, James D Hoyer2, Josiane Bardakdjian-Michau1.
Abstract
OBJECTIVES: In this report, we evaluated utility of the capillary electrophoresis (CE) migration position of the CAPILLARYS 2 CE instrument.
METHODS: The precision of this x-axis number was determined on a selection of common hemoglobin (Hb) variants (Hb S, Hb C, Hb D-Punjab, Hb E, Hb Hope), and the reproducibility of this number was evaluated by comparing the results obtained by two large reference laboratories on 81 Hb variants. Additionally, the CE migration position is given for a total of 409 Hb variants.
RESULTS: The x-axis migration position showed excellent intra- and interassay precision. Comparison of Hb variants seen by both laboratories showed that 83% had a difference in migration position of 1 unit or less. Only three rare Hb variants showed a difference of more than 2 units.
CONCLUSION: In summary, the CE migration position is a reproducible value and can be used as an aid in the identification of Hb variants. © American Society for Clinical Pathology, 2018. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
OBJECTIVES: In this report, we evaluated utility of the capillary electrophoresis (CE) migration position of the CAPILLARYS 2 CE instrument.
METHODS: The precision of this x-axis number was determined on a selection of common hemoglobin (Hb) variants (Hb S, Hb C, Hb D-Punjab, Hb E, Hb Hope), and the reproducibility of this number was evaluated by comparing the results obtained by two large reference laboratories on 81 Hb variants. Additionally, the CE migration position is given for a total of 409 Hb variants.
RESULTS: The x-axis migration position showed excellent intra- and interassay precision. Comparison of Hb variants seen by both laboratories showed that 83% had a difference in migration position of 1 unit or less. Only three rare Hb variants showed a difference of more than 2 units.
CONCLUSION: In summary, the CE migration position is a reproducible value and can be used as an aid in the identification of Hb variants. © American Society for Clinical Pathology, 2018. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Entities:
Keywords:
CAPILLARYS 2; Capillary electrophoresis; Hemoglobinopathies
Mesh：
Substances：
Year: 2018
PMID： 29365076 DOI： 10.1093/ajcp/aqx148
Source DB: PubMed Journal: Am J Clin Pathol ISSN： 0002-9173 Impact factor: 2.493