K Wang1, X-J Qi, H-Z Liu, H Su. 1. Department of Surgery, Yantaishan Hospital, Yantai, China. suhao78@sohu.com.
Abstract
OBJECTIVE: MicroRNAs have been reported to contribute to the development of osteosarcoma via negatively regulating the target genes. Nevertheless, the undiscovered function of miR-361 on osteosarcoma development remains uncertain. PATIENTS AND METHODS: MiR-361 and FKBP14 (FK506-binding protein 14) expression in osteosarcoma samples were detected by Real-time polymerase chain reaction (PCR). Cells invasive ability was examined via the transwell invasion assay. The luciferase reporter assay was used to examine the regulation mechanism. The protein level of FKBP14 was detected by Western blot. Cell Counting Kit-8 (CCK-8) was used to detect cell lines proliferation. RESULTS: MiR-361 was reduced both in osteosarcoma samples and cell lines. Up-regulation of miR-361 significantly inhibited cells invasive and proliferative abilities, while down-regulation of miR-361 promoted cell lines invasion and proliferation. miR-361 could negatively regulate FKBP14 in osteosarcoma. Suppression of FKBP14 could reverse the function of miR-361 inhibitor. CONCLUSIONS: MiR-361 inhibits osteosarcoma cell lines invasion and proliferation by targeting FKBP14.
OBJECTIVE: MicroRNAs have been reported to contribute to the development of osteosarcoma via negatively regulating the target genes. Nevertheless, the undiscovered function of miR-361 on osteosarcoma development remains uncertain. PATIENTS AND METHODS: MiR-361 and FKBP14 (FK506-binding protein 14) expression in osteosarcoma samples were detected by Real-time polymerase chain reaction (PCR). Cells invasive ability was examined via the transwell invasion assay. The luciferase reporter assay was used to examine the regulation mechanism. The protein level of FKBP14 was detected by Western blot. Cell Counting Kit-8 (CCK-8) was used to detect cell lines proliferation. RESULTS:MiR-361 was reduced both in osteosarcoma samples and cell lines. Up-regulation of miR-361 significantly inhibited cells invasive and proliferative abilities, while down-regulation of miR-361 promoted cell lines invasion and proliferation. miR-361 could negatively regulate FKBP14 in osteosarcoma. Suppression of FKBP14 could reverse the function of miR-361 inhibitor. CONCLUSIONS:MiR-361 inhibits osteosarcoma cell lines invasion and proliferation by targeting FKBP14.