| Literature DB >> 29363073 |
Lars F Eggers1, Dominik Schwudke2.
Abstract
Shotgun lipidomics offers fast and reproducible identification and quantification of lipids in clinical samples. Lipid extraction procedures based on the methyl tert-butyl protocol are well established for performing shotgun lipidomics in biomedical research. Here, we describe a shotgun lipidomics workflow that is well suited for the analysis of clinical samples such as tissue samples, blood plasma, and peripheral blood mononuclear cells.Entities:
Keywords: Clinical samples; High-resolution mass spectrometry; Lipid extraction; Lipid identification; Shotgun lipidomics
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Year: 2018 PMID: 29363073 DOI: 10.1007/978-1-4939-7592-1_12
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745