Rie Karasawa1, Mayumi Tamaki1, Toshiko Sato1, Megumi Tanaka1, Makiko Nawa2, Kazuo Yudoh1, James N Jarvis3,4. 1. Department of Frontier Medicine, St Marianna University School of Medicine, Kawasak, Japani. 2. Institute of Nanken-Kyoten and RCC, Tokyo Medical and Dental University, Tokyo, Japan. 3. Department of Pediatrics and Genetics, Genomics, & Bioinformatics Program, Jacobs School of Medicine and Biomedical Sciences University at Buffalo, Buffalo, NY, USA. 4. Genetics, Genomics, & Bioinformatics Program, Jacobs School of Medicine and Biomedical Sciences University at Buffalo, Buffalo, NY, USA.
Abstract
Objective: Although generally classified within the group of inflammatory myopathies, JDM displays many pathological features of vasculitis. Previous work has shown that AECA are abundant in other forms of vasculitis. We therefore investigated whether such antibodies might also be detected in JDM. Methods: We screened plasma from children with JDM for the presence of AECA by western blotting and 2D gel electrophoresis (2DE) using proteins extracted from human aortic endothelial cells as the substrate. We performed mass spectrometry to identify candidate antigens from 2DE gels and used ELISA to confirm the presence of specific antibodies. Results: We identified 22 candidate target autoantigens for AECA probed with JDM plasma. Interestingly, 17 of these 22 target antigens were proteins associated with antigen processing and protein trafficking. ELISA confirmed the presence of antibodies to heat shock cognate 71 kDa protein in JDM plasma, particularly in children with active, untreated disease. Conclusion: Children with JDM express antibodies to autoantigens in endothelial cells. The clinical and pathological significance of such autoantibodies require further investigation.
Objective: Although generally classified within the group of inflammatory myopathies, JDM displays many pathological features of vasculitis. Previous work has shown that AECA are abundant in other forms of vasculitis. We therefore investigated whether such antibodies might also be detected in JDM. Methods: We screened plasma from children with JDM for the presence of AECA by western blotting and 2D gel electrophoresis (2DE) using proteins extracted from human aortic endothelial cells as the substrate. We performed mass spectrometry to identify candidate antigens from 2DE gels and used ELISA to confirm the presence of specific antibodies. Results: We identified 22 candidate target autoantigens for AECA probed with JDM plasma. Interestingly, 17 of these 22 target antigens were proteins associated with antigen processing and protein trafficking. ELISA confirmed the presence of antibodies to heat shock cognate 71 kDa protein in JDM plasma, particularly in children with active, untreated disease. Conclusion:Children with JDM express antibodies to autoantigens in endothelial cells. The clinical and pathological significance of such autoantibodies require further investigation.
Authors: Rie Karasawa; Kazuo Yudoh; Toshiko Sato; Megumi Tanaka; Mayumi Tamaki; Sara E Sabbagh; Terrance P O'Hanlon; Payam Noroozi-Farhadi; Ira N Targoff; Willy A Flegel; Andrew L Mammen; Frederick W Miller; Mark D Hicar; Lisa G Rider; James N Jarvis Journal: Rheumatology (Oxford) Date: 2022-07-06 Impact factor: 7.046
Authors: Judith Wienke; Claire T Deakin; Lucy R Wedderburn; Femke van Wijk; Annet van Royen-Kerkhof Journal: Front Immunol Date: 2018-12-18 Impact factor: 7.561