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Literature DB >> 29359100

Fast epi-detected broadband multiplex CARS and SHG imaging of mouse skull cells.

Erwan Capitaine^1,2, Nawel Ould Moussa¹, Christophe Louot¹, Sylvia M Bardet¹, Hideaki Kano^3,4,5, Ludovic Duponchel⁶, Philippe Lévêque¹, Vincent Couderc¹, Philippe Leproux¹.

Abstract

We present a bimodal imaging system able to obtain epi-detected mutiplex coherent anti-Stokes Raman scattering (M-CARS) and second harmonic generation (SHG) signals coming from biological samples. We studied a fragment of mouse parietal bone and could detect broadband anti-Stokes and SHG responses originating from bone cells and collagen respectively. In addition we compared two post-processing methods to retrieve the imaginary part of the third-order nonlinear susceptibility related to the spontaneous Raman scattering.

Entities: Gene Species

Keywords: (170.3880) Medical and biological imaging; (180.4315) Nonlinear microscopy; (300.6230) Spectroscopy, coherent anti-Stokes Raman scattering

Year: 2017 PMID： 29359100 PMCID： PMC5772578 DOI： 10.1364/BOE.9.000245

Source DB: PubMed Journal: Biomed Opt Express ISSN： 2156-7085 Impact factor: 3.732

17 in total

1. Chemical specificity in three-dimensional imaging with multiplex coherent anti-Stokes Raman scattering microscopy.

Authors: George W H Wurpel; Juleon M Schins; Michiel Müller
Journal: Opt Lett Date: 2002-07-01 Impact factor: 3.776

2. Ultrabroadband multiplex CARS microspectroscopy and imaging using a subnanosecond supercontinuum light source in the deep near infrared.

Authors: Masanari Okuno; Hideaki Kano; Philippe Leproux; Vincent Couderc; Hiro-O Hamaguchi
Journal: Opt Lett Date: 2008-05-01 Impact factor: 3.776

Fast epi-detected broadband multiplex CARS and SHG imaging of mouse skull cells.

1. Chemical specificity in three-dimensional imaging with multiplex coherent anti-Stokes Raman scattering microscopy.

2. Ultrabroadband multiplex CARS microspectroscopy and imaging using a subnanosecond supercontinuum light source in the deep near infrared.

Review 3. Contributions of Raman spectroscopy to the understanding of bone strength.

4. Multicomponent chemical imaging of pharmaceutical solid dosage forms with broadband CARS microscopy.

5. Raman spectroscopic monitoring of the osteogenic differentiation of human mesenchymal stem cells.

6. Carbonate assignment and calibration in the Raman spectrum of apatite.

7. The anti-apoptotic gene survivin contributes to teratoma formation by human embryonic stem cells.

8. Raman spectroscopy and CARS microscopy of stem cells and their derivatives.

9. Vibrational microscopy and imaging of skin: from single cells to intact tissue.

10. Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells.

1. Multiplex coherent anti-Stokes Raman scattering highlights state of chromatin condensation in CH region.

2. Coherent anti-Stokes Raman scattering cell imaging and segmentation with unsupervised data analysis.

3. Murine Metatarsus Bone and Joint Collagen-I Fiber Morphologies and Networks Studied With SHG Multiphoton Imaging.