Literature DB >> 29357110

Hybridization Histochemistry of Neural Transcripts.

W Scott Young1, June Song1, Éva Mezey2.   

Abstract

This unit presents protocols to locate RNA transcripts in tissues. Numerous approaches are detailed, including those that use radiolabeled or colorimetric probes. Also, the probes may be modified oligodeoxynucleotides, singly or in pairs, as well as ribonucleic acids. High sensitivity and specificity are obtained, especially with sets of oligodeoxynucleotide pairs. © 2018 by John Wiley & Sons, Inc.
Copyright © 2018 John Wiley & Sons, Inc.

Entities:  

Keywords:  ViewRNA; digoxigenin; in situ hybridization; mRNA; riboprobes

Mesh：

Substances：

Year:  2018        PMID： 29357110      PMCID： PMC6217960          DOI： 10.1002/cpns.39

Source DB:  PubMed          Journal:  Curr Protoc Neurosci        ISSN： 1934-8576


  23 in total

1.  Spatial and temporal expression of alpha- and beta-thyroid hormone receptor mRNAs, including the beta 2-subtype, in the developing mammalian nervous system.

Authors:  D J Bradley; H C Towle; W S Young
Journal:  J Neurosci       Date:  1992-06       Impact factor: 6.167

2.  An in situ hybridization histochemistry method for the use of alkaline phosphatase-labeled oligonucleotide probes in small intestine.

Authors:  H Kiyama; P C Emson
Journal:  J Histochem Cytochem       Date:  1991-10       Impact factor: 2.479

3.  Double immunolabeling of neuropeptides in the human hypothalamus as analyzed by confocal laser scanning fluorescence microscopy.

Authors:  H J Romijn; J F van Uum; I Breedijk; J Emmering; I Radu; C W Pool
Journal:  J Histochem Cytochem       Date:  1999-02       Impact factor: 2.479

4.  Molecular cloning and sequence analysis of a mouse Y chromosome RNA transcript expressed in the testis.

Authors:  C E Bishop; D Hatat
Journal:  Nucleic Acids Res       Date:  1987-04-10       Impact factor: 16.971

5.  Simultaneous visualization of multiple antigens with tyramide signal amplification using antibodies from the same species.

Authors:  Zsuzsanna E Tóth; Eva Mezey
Journal:  J Histochem Cytochem       Date:  2007-01-22       Impact factor: 2.479

Review 6.  Fluorescence in situ hybridization for the detection of DNA and RNA.

Authors:  D G Albertson; R M Fishpool; P S Birchall
Journal:  Methods Cell Biol       Date:  1995       Impact factor: 1.441

7.  A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine.

Authors:  H M Kerstens; P J Poddighe; A G Hanselaar
Journal:  J Histochem Cytochem       Date:  1995-04       Impact factor: 2.479

8.  Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays.

Authors:  M N Bobrow; T D Harris; K J Shaughnessy; G J Litt
Journal:  J Immunol Methods       Date:  1989-12-20       Impact factor: 2.303

9.  Transplanted bone marrow generates new neurons in human brains.

Authors:  Eva Mezey; Sharon Key; Georgia Vogelsang; Ildiko Szalayova; G David Lange; Barbara Crain
Journal:  Proc Natl Acad Sci U S A       Date:  2003-01-21       Impact factor: 11.205

10.  The distribution of thalamic projection neurons containing cholecystokinin messenger RNA, using in situ hybridization histochemistry and retrograde labeling.

Authors:  J M Burgunder; W S Young
Journal:  Brain Res       Date:  1988-11       Impact factor: 3.252
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  2 in total

1.  Detection and Quantification of Multiple RNA Sequences Using Emerging Ultrasensitive Fluorescent In Situ Hybridization Techniques.

Authors:  Larissa Erben; Andres Buonanno
Journal:  Curr Protoc Neurosci       Date:  2019-02-21

2.  NMDA Receptor in Vasopressin 1b Neurons Is Not Required for Short-Term Social Memory, Object Memory or Aggression.

Authors:  Sarah K Williams Avram; Heon-Jin Lee; Jarrett Fastman; Adi Cymerblit-Sabba; Adam Smith; Matthew Vincent; June Song; Michael C Granovetter; Su-Hyun Lee; Nicholas I Cilz; Michelle Stackmann; Rahul Chaturvedi; W Scott Young
Journal:  Front Behav Neurosci       Date:  2019-11-08       Impact factor: 3.558
  2 in total

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