| Literature DB >> 29355756 |
Subhadeep Roy1, Manjari Singh1, Atul Rawat2, Uma Devi3, Swetlana Gautam1, Rajnish Kumar Yadav1, Jitendra Kumar Rawat1, Md Nazam Ansari4, Abdulaziz S Saeedan4, Dinesh Kumar2, Gaurav Kaithwas5.
Abstract
The aim of the present study is to evaluate the effect of gamma linolenic acid (GLA) on mitochondrial mediated death apoptosis, hypoxic microenvironment and cholinergic anti-inflammatory pathway against 7, 12-dimethylbenz (a) anthracene (DMBA) induced mammary gland carcinoma. The effects of GLA were evaluated morphologically and biochemically against DMBA induced mammary gland carcinoma. The metabolic study was done for evaluation of biomarkers using 1H NMR. The present study was also verified through immunoblotting and qRT-PCR studies for the evaluation of various pathways. GLA treatment has a delineate implementation upon morphology of the tissues when evaluated through carmine staining, hematoxyline and eosin staining and scanning electron microscopy. GLA also demarked a commendatory proclamation of the fifteen key serum metabolites analogous with amino acid metabolism and fatty acid metabolism when recognized through1H NMR studies. The immunoblotting and qRT-PCR studies accomplished that GLA mediated mitochondrial death apoptosis, curtail hypoxic microenvironment along with hindrance of de novo fatty acid synthesis and also mediate the cholinergic anti-inflammatory pathway to proclaim its anticancer effects.Entities:
Keywords: 7, 12-Dimethylbenz (a) anthracene; Breast cancer; Gamma linolenic acid; Hypoxia; Mitochondria mediated death apoptosis; alpha-7-nachr
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Year: 2018 PMID： 29355756 DOI： 10.1016/j.biocel.2018.01.011
Source DB: PubMed Journal: Int J Biochem Cell Biol ISSN： 1357-2725 Impact factor: 5.085