| Literature DB >> 2935423 |
Y Le Bouc, D Dreyer, F Jaeger, M Binoux, P Sondermeyer.
Abstract
A full-size cDNA sequence coding for insulin-like growth factor I (IGF-I) was isolated from a human liver library. For the construction of this bank, a new method was developed which anneals dG-tailed cDNA with a synthetic adaptor 5'-AATTCCCCCCCCCCC-3' followed by ligation into the EcoRI site of a lambda immunity-insertion vector. Based on the sequence analysis of the complete IGF-I messenger we concluded that the protein is synthesized as a precursor containing a signal peptide of 22 or 25 amino acid residues. In addition, the sequence extended in the 3'-direction and showed the presence of multiple polyadenylation sites in the IGF-I message.Entities:
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Year: 1986 PMID： 2935423 DOI： 10.1016/0014-5793(86)80223-x
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124